FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n02_ITS-OOATH575.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n02_ITS-OOATH575.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7361
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT520870.75125662274148No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT140919.141421002581172No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT260.35321287868496126No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT230.3124575465290042No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAGAGACT220.29887243581035183No Hit
AGCCTAGACATCAACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT180.24453199293574243No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGAAT150.20377666077978535No Hit
AGCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACTT130.17660643934248063No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGGACT120.1630213286238283No Hit
AGCCTAGACATCCACTGATGAAAGTTGTTATCTAGTTAAAAGCAGAGACT120.1630213286238283No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT120.1630213286238283No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT110.14943621790517592No Hit
AGCCTAGACATCCACTGTTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT100.13585110718652357No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACC100.13585110718652357No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTTAAAAGCAGAGACT100.13585110718652357No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT100.13585110718652357No Hit
AGCATAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT90.12226599646787122No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT90.12226599646787122No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAGAGACT80.10868088574921884No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTACAAGCAGAGACT80.10868088574921884No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGGAGACT80.10868088574921884No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)