FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n01_ITS-OOATH911.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n01_ITS-OOATH911.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5448
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT508293.28193832599119No Hit
CGGAAGGATCATTACCACACAAAAAACTATCCACGTGAACCACTTTGTAT400.7342143906020557No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACAACTTTGTAT360.6607929515418502No Hit
CGGAAGGATCATTACCACACCAAAAACTATACACGTGAACCACTTTGTAT300.5506607929515419No Hit
CGGAAGGATCATTAACACACCAAAAACTATCCACGTGAACCACTTTGTAT290.5323054331864905No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCACTTTGTAT90.16519823788546256No Hit
TGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT60.11013215859030838No Hit
CGGAAGGATCATTACCACATCTCCGAGCAGCAGTGGATGTCTAGGCTCTG60.11013215859030838No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCAATTTGTAT60.11013215859030838No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)