FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n01_ITS-OOATH663.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n01_ITS-OOATH663.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5897
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC316253.620484992369No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT153325.99626928946922No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTTCCACGTGAACCGTATCAA1472.492792945565542No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCGTTAAGC971.6449041885704594No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACTGTCGTTAT701.187044259793115No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTCCACGTGAACTGTATCAAA470.7970154315753774No Hit
CGGAAGGATCATTAACACACCTAAAAACTTTCCACGTGAACCGTATCAAC430.7291843310157707No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAAACGTATCAAC350.5935221298965575No Hit
CGGAAGGATCATTACCACACATAAAAACTTTCCACGTGAACCGTATCAAC340.5765643547566559No Hit
CGGAAGGATCATTACCACACCAAAAACAATCCACGTGAACCGTCAGAAAT310.525691029336951No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCACTTTGTA280.47481770391724604No Hit
CGGAAGGATCATTACCACGAGACCAGCAGTGGATGTCTAGGCTCTGTCTC260.44090215363744273No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACAACTTTGTAT220.37307105307783617No Hit
CGGAAGGATCATTACCACACCTAAAAAATTTCCACGTGAACCGTATCAAC210.35611327793793457No Hit
CGGAAGGATCATTACCACACAAAAAACTATCCACGTGAACCACTTTGTAT190.32219772765813126No Hit
CGGAAGGATCATTACCACACCAAAAACTATACACGTGAACCACTTTGTAT160.27132440223842635No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTGAACCACTTTGTAT160.27132440223842635No Hit
CGGAAGGATCATTAACACACCAAAAACTATCCACGTGAACCACTTTGTAT140.23740885195862302No Hit
CGGAAGGATCATTACCACGCCTAAAAACTTTCCACGTGAACCGTATCAAC120.2034933016788197No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTATCAACA100.16957775139901646No Hit
CGGAAGGATCATTACCAAACCTAAAAACTTTCCACGTGAACCGTATCAAC100.16957775139901646No Hit
CGGAAGGATCATTACCACACCAAAAAAAACTTTCCACGTGAACCGTTGTA90.1526199762591148No Hit
CGGAAGGATCATTACCACACCTAAAAACTATCCACGTGAACCACTTTGTA80.13566220111921318No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATTAAC80.13566220111921318No Hit
CGGAAGGATTATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC80.13566220111921318No Hit
CGGAAGGATCATTACCACGTCGTCGGCAGCAGTGGATGTCTAGGCTCTGT80.13566220111921318No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTATCAACC70.11870442597931151No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAGCCGTATCAAC70.11870442597931151No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCACTTTGT70.11870442597931151No Hit
TGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC70.11870442597931151No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAGCCACTTTGTAT70.11870442597931151No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAGC60.10174665083940986No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTTAACCACTTTGTAT60.10174665083940986No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCGTATCAA60.10174665083940986No Hit
CGGAAGGATCATTATCACACCTAAAAACTTTCCACGTGAACCGTATCAAC60.10174665083940986No Hit
TGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT60.10174665083940986No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCGCGTGAACCGTATCAAC60.10174665083940986No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)