FastQCFastQC Report
Tue 16 Jan 2024
000000000-LC922_l01_n01_ITS-OOATH595.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LC922_l01_n01_ITS-OOATH595.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7425
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC573277.1986531986532No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT7339.872053872053872No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCACTTTGTA460.6195286195286196No Hit
CGGAAGGATCATTACCACACATAAAAACTTTCCACGTGAACCGTATCAAC390.5252525252525253No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAAACGTATCAAC350.47138047138047134No Hit
CGGAAGGATCATTAACACACCTAAAAACTTTCCACGTGAACCGTATCAAC310.41750841750841755No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTGAACCACTTTGTAT290.39057239057239057No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAGCCGTATCAAC270.36363636363636365No Hit
CGGAAGGATCATTACCACACCTAAAAACCTTCCACGTGAACCGTATCAAC220.2962962962962963No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGCGAACCGTATCAAC200.26936026936026936No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGGACCGTATCAAC170.22895622895622897No Hit
CGGAAGGATCATTACCACACCTAAAAACTCTCCACGTGAACCGTATGAAC160.21548821548821548No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTACCAAC160.21548821548821548No Hit
CGGAAGGATCATTACCACACCTAAAAACTCTCCACGTGAACCGTATCAAC150.20202020202020202No Hit
CGGAAGGATCATTACCACGCCTAAAAACTTTCCACGTGAACCGTATCAAC150.20202020202020202No Hit
CGGAAGGATCATTACCACACCTAGAAACTTTCCACGTGAACCGTATCAAC150.20202020202020202No Hit
CGGAAGGATCATTACCACACCTAAAAAATTTCCACGTGAACCGTATCAAC150.20202020202020202No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAGC130.1750841750841751No Hit
CGGAAGGATTATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC130.1750841750841751No Hit
CGGAAGGATCATTACCACACAAAAAACTATCCACGTGAACCACTTTGTAT120.1616161616161616No Hit
CGGAAGGATCATTACCACCTAAAAACTTTCCACGTGAACCGTATCAACAA120.1616161616161616No Hit
CGGAAGGATCATTACCACACCTAAAAACTTCCCACGTGAACCGTATCAAC120.1616161616161616No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTATCAACA110.14814814814814814No Hit
CGGAAGGATCATACCACACCTAAAAACTTTCCACGTGAACCGTATCAACA110.14814814814814814No Hit
CGGAAGGATCATTACCACACCTAAAAACTATCCACGTGAACCACTTTGTA110.14814814814814814No Hit
TGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC110.14814814814814814No Hit
CGGAAGGTCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAACA100.13468013468013468No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCGAC100.13468013468013468No Hit
CGGAAGGATCATTACCACACCCAAAAACTTTCCACGTGAACCGTATCAAC100.13468013468013468No Hit
CGGAAGGATCATTACCACACCTAAAGACTTTCCACGTGAACCGTATCAAC90.12121212121212122No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGCATCAAC90.12121212121212122No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCGCGTGAACCGTATCAAC90.12121212121212122No Hit
CGGAAGGATCATTACCACACCTAAAAGCTTTCCACGTGAACCGTATCAAC90.12121212121212122No Hit
CGGAAGGATCATTACCACACCTAAAACTTTCCACGTGAACCGTATCAACA80.10774410774410774No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACATGAACCGTATCAAC80.10774410774410774No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACTGTATCAAC80.10774410774410774No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)