FastQCFastQC Report
Sat 17 Feb 2024
000000000-LBY9B_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LBY9B_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10282613
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC232898722.649758383399238Illumina Single End PCR Primer 1 (100% over 8bp)
CTAGTATG3934853.8267024150378894No Hit
TCTACACT3775183.6714208732741374Illumina Single End PCR Primer 1 (100% over 8bp)
GATAGCGT3389903.29673012103052No Hit
GTCTAGTG3288063.197689147690378No Hit
CGTGAGTG3073562.989084583850428No Hit
ACGCGTGA3046952.9632059477488846No Hit
GGATATCT3035602.9521678974011762No Hit
GACACCGT2762232.6863113490705133No Hit
CGATCTAC2589112.5179494745158646No Hit
TCATCGAG2512622.443561767811353No Hit
GTCAGATA2453552.386115280230813No Hit
AAGCAGCA2386402.3208108678212436No Hit
ACGTCTCG2246782.1850282608126945No Hit
TGCGTCAC2234882.1734553269679604No Hit
CGTTACTA2226122.1649360916335176No Hit
TCGACGAG2137472.0787225970674963No Hit
GATCGTGT1997521.9426190599607318No Hit
TAGCGAGT1953541.8998478305076734No Hit
CTACTATA1950971.8973484658033908No Hit
TGCGTACG1892501.840485487492333No Hit
CTAGAGCT1855111.8041231348490894No Hit
CTGCGTGT1822211.7721273765724723No Hit
ACTATCTG1759681.7113159855379174No Hit
TAGTGTAG1756181.7079121814659366No Hit
CGTCGCTA1675271.6292259564762384No Hit
TACGAGAC1655251.6097561971845094TruSeq Adapter, Index 14 (100% over 8bp)
GACACTGA1643791.598611170137396No Hit
GCTCTAGT1594941.551103790447039No Hit
ATCGTACG1520071.4782915587701297RNA PCR Primer, Index 22 (100% over 8bp)
AGAGTCAC1452091.4121799585377763No Hit
ACGACGTG1444001.4043123085542557No Hit
ATATACAC1428611.3893452957920327No Hit
GTAAGGAG117360.11413441311075308No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)