FastQCFastQC Report
Sat 17 Feb 2024
000000000-LBY9B_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LBY9B_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10282613
Sequences flagged as poor quality0
Sequence length8
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCACTG3242873.1537411745438635No Hit
GACATAGT3015192.932318857084284No Hit
TAGTCTCC2924162.843790775749316No Hit
TCTCTATG2826882.7491844728572397ABI Solid3 Adapter B (100% over 8bp)
CGAGAGTT2777432.7010935838974004TruSeq Adapter, Index 13 (100% over 8bp)
CGAGCGAC2513632.4445440084149817No Hit
CGAAGTAT2471112.403192651517664No Hit
TGAGTACG2280712.218025710001923No Hit
CTGCGTAG2131372.0727902528277586No Hit
ACGTACGT2059912.0032942988324076No Hit
TACTAGGT2032331.976472322745201No Hit
CTCGACTT2019521.9640143998417525No Hit
ACGTGCGC1983071.9285662117206979No Hit
GATCTACG1978231.9238592369468732No Hit
AGTCGCAG1930431.8773729984781107No Hit
GTAACGAG1911571.859031357107381No Hit
AACGCTGA1886191.8343489150082766No Hit
TAGCAGCT1775051.7262635479911574No Hit
GTTACAGC1704821.6579637879982452No Hit
ACTACGAC1666941.6211249027849244No Hit
ATAGTACC1626481.5817769277128295No Hit
ACGCTACT1623161.5785481764216938No Hit
GCGATACG1441221.4016087156056538No Hit
CGTAGCGA1407441.368757143733796No Hit
GTCTATGA1380741.3427909812418304No Hit
GTCTGCTA1379521.3416045123938827No Hit
CTATCGTG1369861.3322100131552166No Hit
TATAGCGA1369131.3315000768773462No Hit
TCAGTCTA1228771.1949978084364354No Hit
CAGTAGGT1188981.1563014187152623No Hit
CATCGTGA1180451.1480058619341211No Hit
GCGTATAC1131481.1003817803898677No Hit
TGCTCGTA1084911.0550917359235439No Hit
TCTAGACT999110.9716499103875639No Hit
CGTCATAC986450.9593378648014859No Hit
ACTGTGTA944900.9189298478898312No Hit
CTACGACC889150.8647121116004269No Hit
GAGACTTA852330.8289040927631917No Hit
ATAGCGCT837080.814073232163848No Hit
ATGAGCTC807650.7854521024957373No Hit
AGCATACC804650.7825345561483253No Hit
TAACGTCC756800.7359996919071057No Hit
CGAGCTAG726430.706464397716806No Hit
TCCTCATG690750.6717650464915873No Hit
CGCGATAT609160.5924175109964753No Hit
TGCGTCAA602870.5863003888214018No Hit
GAGCTCGA552260.5370813819405631No Hit
CTCTAGAG403760.39266283774367466No Hit
ACGTATAC198010.192567784083676No Hit
AACATAGT168660.16402445565149637No Hit
ATAACGAG162800.1583255151195518No Hit
ACGATACG140490.13662869544929873No Hit
AAAAAAAA127000.12350946204043661No Hit
GGACTCCT114210.11107098944597059No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)