FastQCFastQC Report
Sat 27 Apr 2024
000000000-LBY8V_l01_n01_JN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LBY8V_l01_n01_JN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGCTCATCTCGTAT22.564102564102564TruSeq Adapter, Index 2 (97% over 38bp)
AGAGACCTTTTCGACGCTGCCGCCACCGCCTTCCCTGTCGCCAGCGCCAC11.282051282051282No Hit
GAGCGCTGCGCGGCAATGTCCGGGCGTTTACCCAGCAAGTCCAGCGTTAA11.282051282051282No Hit
CCCAACTCGCCTTGATCCTGAATCTGCAAACCATGGCCGAGCAATTCGCT11.282051282051282No Hit
AGAAGTTAATGGATGAATTGGCACAATGCTACAATGTGCTCCCCCAACTT11.282051282051282No Hit
ATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCGTCCTGCGTGTAGC11.282051282051282No Hit
GAAGCTGGAGTAACAGAAGTGAGAACCAGCTTATCAGAAAAAAAGTTTGA11.282051282051282No Hit
ACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGAT11.282051282051282No Hit
TCCCCAATGCTTGGCTTCCATAAGCAGATGGATAACCGCATCAAGCTCTT11.282051282051282No Hit
CCCACGGCAGGCTGTGCGTCGCGTCACTTCTGGCCTCTGGCAACGTACCG11.282051282051282No Hit
CGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGG11.282051282051282No Hit
TTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGC11.282051282051282No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGCTCATATCGTAT11.282051282051282TruSeq Adapter, Index 2 (97% over 38bp)
AGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTA11.282051282051282No Hit
GTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGC11.282051282051282No Hit
GTACGGGGAAGGACGTCAATAGTCACACAGTCCTTGACGGTATAATAACC11.282051282051282No Hit
GGGCTACTCCAAATGGCACCTGCAGCGCATGTTTAAAGAGCACACCGGTT11.282051282051282No Hit
CAAGCGAAGCGCGGTAGGTTTTCTGCTTAGGAGTTTAATCATGTTTCAGA11.282051282051282No Hit
ATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGCTATTGCT11.282051282051282No Hit
TAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTTCG11.282051282051282No Hit
CTTCCAGCACGTTCAGGATCTCAGCATCAGCAATCAGGTTGATGGTGGTT11.282051282051282No Hit
ACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAGCGCAAGAGTA11.282051282051282No Hit
TGTAAAACAGGTGCCGAAGAAGCTGGAGTAACAGAAGTGAGAACCAGCTT11.282051282051282No Hit
GCTCTAATGTTCCTAACCCTGATGAGGCCGTCCCTAGTTTTGTTTCTGGT11.282051282051282No Hit
TCAGTAGCAATCCAAACTTTGTTACTCGTCAGAAAATCGAAATCATCTTC11.282051282051282No Hit
TTGTTATCGGTAGCAAGCACATCACCTTGAATGCCACCGGAGGCGGCTTT11.282051282051282No Hit
AAACCTACCGCGCTTCGCTTGGTCAACCCCTCAGCGGCAAAAATTAAAAT11.282051282051282No Hit
AGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTGTCCACGGA11.282051282051282No Hit
CAAAACCACGATCGCATTTCGACCGAGAACACGCATTTTTTGCCGCGCTT11.282051282051282No Hit
CAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCC11.282051282051282No Hit
CAACCTGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAAT11.282051282051282No Hit
AAAAAAAGACTTAAACATAGGCACTCCGATGTTGCGTGTTGCCTGTTATA11.282051282051282No Hit
AAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATTTTAATTT11.282051282051282No Hit
GACGATGTAGCTTTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGAGT11.282051282051282No Hit
CCTGACTATTCCACTGCAACAACTGAACGGACTGGAAACACTGGTCATAA11.282051282051282No Hit
TTGATTAAGCTCATTAGGGTTAGCCTCGGTACGGTCAGGCATCCACGGCG11.282051282051282No Hit
GGGATGAAAATGCTCACAATGACAAATCTGTCCACGGAGTGCTTAATCCA11.282051282051282No Hit
CACAGGTTGCGCCGCCAAAACGTCGGCTACAGTAACTTTTCCCAGCCTCA11.282051282051282No Hit
TTAAGTGGCTGGAGACAAATAATCTCTTTAATAACCTGATTCAGCGAAAC11.282051282051282No Hit
ACGAGGAAAGTCGGCAAGTCTAACCGATGCCGCAGGCAATCATCGAATTG11.282051282051282No Hit
AAACAAATGCTTAGGGATTTTATTGGTATCAGGGTTAATCGTGCCAAGAA11.282051282051282No Hit
CTTTATCAAGATAATTTTTCGACTCATCAGAAATATCCGAAAGTGTTAAC11.282051282051282No Hit
TGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAG11.282051282051282No Hit
CTGCGATGGGCATACTGTAACCATAAGGCCACGTATTTTGCAAGCTATTT11.282051282051282No Hit
TGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTC11.282051282051282No Hit
CTAACCCTAATGAGCTTAATCAAGATGATGCTCGTTATGGTTTCCGTTGC11.282051282051282No Hit
CGGGCACCAGTCGGGACTCAATCTCAAAAGGGCTGCTGATTGCGCTGCCG11.282051282051282No Hit
CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAATAATG11.282051282051282No Hit
CCGACTGCCTATGATGTTTATCCTTTGGATGGTCGCCATGATGGTGGTTA11.282051282051282No Hit
CCCATGCCTACAGTATTGTTATCGGTAGCAAGCACATCACCTTGAATGCC11.282051282051282No Hit
ATGGCGACCATCCAAAGGATAGACATCATAGGCAGTCGGGAGGGTAGTCG11.282051282051282No Hit
CGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAGAAAAACAATCAAC11.282051282051282No Hit
CGTATCCAACCTGCAGAGTTTTATCGCTTCCATGACGCAGAAGTTAACAC11.282051282051282No Hit
CCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAG11.282051282051282No Hit
TGAAAATGCTCACAATGACAAATCTGTCCACGGAGTGCTTAATCCAACTT11.282051282051282No Hit
CCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACG11.282051282051282No Hit
GTCAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTTTGGCCTCTATT11.282051282051282No Hit
CACGTATTTTGCAAGCTATTTAACTGGCGGCGATTGCGTACCCGACGACC11.282051282051282No Hit
TATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGACC11.282051282051282No Hit
AAAAATATCAACCACACCAGAAGCAGCATCAGTGACGACATTAGAAATAT11.282051282051282No Hit
ATAGACGCAACGCGAGCAGTAGACTCCTTCTGTTGATAAGCAAGCATCTC11.282051282051282No Hit
ACTAACCTTGCGAGTCATTTCTTTGATTTGGTCATTGGTAAAATACTGAC11.282051282051282No Hit
TTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTGCTA11.282051282051282No Hit
ATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTT11.282051282051282No Hit
GCATGAAGTAATCACGTTCTTGGTCAGTATGCAAATTAGCATAAGCAGCT11.282051282051282No Hit
AAAATACTGACCAGCCGTTTGAGCTTGAGTAAGCATTTGGCGCATAATCT11.282051282051282No Hit
ACATCACCTTGAATGCCACCGGAGGCGGCTTTTTGACCGCCTCCAAACAA11.282051282051282No Hit
GTGCCAAGCATATTAAGCCACTTCTCCTCATCCAACGCGTCAGTTTTTGA11.282051282051282No Hit
TGACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTA11.282051282051282No Hit
GCATCAGTGACGACATTAGAAATATCCTTTGCAGTAGCGCCAATATGAGA11.282051282051282No Hit
GTCTGCAAGCTGCTTATGCTAATTTGCATACTGACCAAGAACGTGATTAC11.282051282051282No Hit
TACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTA11.282051282051282No Hit
CCTGATTAGCGGCGTTGACAGATGTATCCATCTGAATGCAATGAAGAAAA11.282051282051282No Hit
TTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGTTTA11.282051282051282No Hit
ATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGC11.282051282051282No Hit
AGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACA11.282051282051282No Hit
ATCCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGAACCGAAG11.282051282051282No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)