FastQCFastQC Report
Wed 15 Nov 2023
000000000-LBVRL_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LBVRL_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461779
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAGGACATCTCGTAT79661.7250676189259364TruSeq Adapter, Index 2 (97% over 38bp)
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT69601.5072144900482698No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGTTAATCTCGTAT48661.0537508201975405TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAACAATCTCGTAT43600.9441745943405829TruSeq Adapter, Index 9 (97% over 38bp)
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG22990.4978572000892202No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGACATCTCGTATG21890.4740362814246642TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAGACATCTCGTATG18650.40387284826724473TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGGACATCTCGTATG16010.3467026434723104TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGACATCTCGTATG14400.311837480699642TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTACCGAAGGACATCTCGTATG12120.2624632129221987Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAGGTCATCTCGTAT10760.23301189530056587TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAGGAAATCTCGTAT9530.2063757771574714TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACGGACATCTCGTAT8940.19359910260102778TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGGTAATCTCGTAT8780.19013424170436508TruSeq Adapter, Index 9 (97% over 37bp)
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG7810.16912852251834753No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGGAAATCTCGTAT7060.15288698706524118TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGGACATCTCGTAT6740.1459572652719158TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGATAATCTCGTAT6550.14184274295712884TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCGTCATCTCGTAT6470.1401103125087975TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGGACATCTCGTAT5990.12971572981880944TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAGGCCATCTCGTAT5990.12971572981880944TruSeq Adapter, Index 2 (97% over 38bp)
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA5840.12646742272818817No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCGTTAATCTCGTATG5530.11975425474090419TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACAACAATCTCGTAT5470.1184549319046557TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATAGTTAATCTCGTAT5360.11607284003820009TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGGTCATCTCGTAT5230.11325764055966166TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGTTCATCTCGTAT5090.11022588727508181TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAACCATCTCGTAT5060.10957622585695756TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGGACATCTCGTAT5050.10935967205091612TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTAATCTCGTATG5020.10871001063279188TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCGAAGGACATCTCGTATG4870.1054617035421706TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAGGCATCTCGTATG4690.10156373503342507TruSeq Adapter, Index 2 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)