FastQCFastQC Report
Wed 15 Nov 2023
000000000-LBVRL_l01_n01_4JT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LBVRL_l01_n01_4JT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095576
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAACAATCTCGTAT36106532.95663650901444TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAACAATATCGTAT42360.38664592871694886TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAACAATTTCGTAT39020.35615968221282684TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAATAATCTCGTAT28480.2599545809692801TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAACAACCTCGTAT16360.1493278421579151TruSeq Adapter, Index 9 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAACAATCTCGTA13000.1186590432795169TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACAACAATCTCGTAT12530.11436906248402667TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAACCATCTCGTAT12490.11400395773547431TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGACAATCTCGTAT11380.10387230096314633TruSeq Adapter, Index 9 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)