FastQCFastQC Report
Wed 15 Nov 2023
000000000-LBVRL_l01_n01_119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-LBVRL_l01_n01_119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140966
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGTTAATCTCGTAT43541920.337501856638546TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGTTAATATCGTAT49860.2328855292424074TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGTTAATTTCGTAT48070.22452481730209634TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGGTAATCTCGTAT32190.1503526912617949TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGATAATCTCGTAT23020.10752155802567626TruSeq Adapter, Index 9 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)