FastQCFastQC Report
Thu 16 Nov 2023
000000000-L9KNV_l01_n01_612.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L9KNV_l01_n01_612.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329495
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCCGGATCTCGTAT24632810.574309024058863TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCCGGATATCGTAT66710.2863710804273029TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCCGGATCTCGCAT62030.2662808892056004TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCCGGATCTTGTAT48710.20910111418998537TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCCGGATCTCGGAT39380.16904951502364246TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCCGGATTTCGTAT30190.1295989044835898TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCCGGATCCCGTAT28900.12406122356991536TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCCGGACCTCGTAT24580.10551643167295917TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGCCGGATCTCGTAA23460.10070852266263718TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)