FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OODve918.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OODve918.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT36718.35No Hit
AGCCTAGACATCCACTGCTGTCTGTGGTAATGATCCTTCCGCTGTCTCTT29814.899999999999999No Hit
AGCCTAGACATCCACTGCTGTGTGGTAATGATCCTTCCGCTGTCTCTTAT1467.3No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC1185.8999999999999995No Hit
AGCCTAGACATCCACTGCTGCCGACGGTGGTAATGATCCTTCCGCTGTCT1005.0No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC603.0No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT552.75No Hit
AGCCTAGACATCCACTGCTGTGGTCGGTGTGGTAATGATCCTTCCGCTGT412.0500000000000003No Hit
AGCCTAGACATCCACTGCTGACGCTGCCGACGAGTGGTAATGATCCTTCC412.0500000000000003No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT341.7000000000000002No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT281.4000000000000001No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT271.35No Hit
AGCCTAGACATCCACTGCTGCTCGGAGATGTGGTAATGATCCTTCCGCTG180.8999999999999999No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGGCTCGGAGATGTGGTAATGATC170.8500000000000001No Hit
AGCCTAGACATCCACTGCTGGTGTCTCGTGGTAATGATCCTTCCGCTGTC140.7000000000000001No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGGCTCGGAGATGTGGTAATGAT130.65No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT120.6No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC110.5499999999999999No Hit
AGCCTAGACATCCACTGCTGCGGTGGTAATGATCCTTCCGCTGTCTCTTA100.5No Hit
AGCCTAGACATCCACTGCTGGAGATGTGGTAATGATCCTTCCGCTGTCTC100.5No Hit
AGCCTAGACATCCACTGCTGTCTGTGGTAATGATACTTCCGCTGTCTCTT100.5No Hit
AGCCTAGACATCCACTGCTGGTGTCTCGTGGGCTCGGAGATGTGGTAATG90.44999999999999996No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT80.4No Hit
AGCCTAGACATCCACTGCTGTCTGTGGTAATGATGCTTCCGCTGTCTCTT70.35000000000000003No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGAACCTTCCGCTGTCTCT70.35000000000000003No Hit
AGCCTAGACATCCACTGCTGTCCCGTGGTAATGATCCTTCCGCTGTCTCT60.3No Hit
AGCCTAGACATCCACTGCTGTCTGTGGTAATGATTCTTCCGCTGTCTCTT60.3No Hit
AGCCTAGACATCCACTGCTGTCCGTGGTAATGATCCTTCCGCTGTCTCTT50.25No Hit
AGCCTAGACATCCACTGCTGTCTCTTATACACATCTGACGCTGCCGACGA40.2No Hit
AGCCTAGACATCCACTGCTGTCTGTGGTAATGATCCTTCCGCTGTCTATT30.15No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGCAGAGAC30.15No Hit
AGCCTAGACATCCACTGCTGCCGACGGTGGTAATTATCCTTCCGCTGTCT30.15No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTACTGATCCTTCCGCTGTCTCT30.15No Hit
AGCCTAGACATCCACTGCTGATGTGTATAAGAGACAGTGGTAATGATCCT30.15No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC30.15No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT30.15No Hit
AGCCTAGACATCCACTGCTGCCTGTGGTAATGATCCTTCCGCTGTCTCTT30.15No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC30.15No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)