FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OODve847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OODve847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC150640.2028830752803No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT2797.447944474105713No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC2707.207688200747464No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT1684.484783769353977No Hit
AGCCTAGACATCCACTGCTGCCGAGTGGTAATGATCCTTCCGCTGTCTCT1423.7907100907634814No Hit
AGCCTAGACATCCACTGCTGCTCCGTGGTAATGATCCTTCCGCTGTCTCT1313.4970635344367325No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAAGCTT1293.443673251468233No Hit
AGCCTAGACATCCACTGCTGGACAGTGGTAATGATCCTTCCGCTGTCTCT1183.1500266951414844No Hit
AGCCTAGACATCCACTGCTGGGTCTCGTGGTAATGATCCTTCCGCTGTCT1143.043246129204485No Hit
AGCCTAGACATCCACTGCTGCCGACGTGGTAATGATCCTTCCGCTGTCTC631.6817939135077415No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT541.4415376401494928No Hit
AGCCTAGACATCCACTGCTGCGGGCAGCGTCAGATGTGGTAATGATCCTT210.5605979711692471No Hit
AGCCTAGACATCCACTGCTGGGCCGGAGATGTGGTAATGATCCTTCCGCT200.5339028296849974No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC130.34703683929524826No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC110.2936465563267485No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC110.2936465563267485No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGGCTCGGAGATGTGGTAATGATC110.2936465563267485No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC100.2669514148424987No Hit
AGCCTAGACATCCACTGCTGTGTCTCGTGGTAATGATCCTTCCGCTGTCT80.21356113187399892No Hit
AGCCTAGACATCCACTGCTGGGTCTCGTGGTAATAATCCTTCCGCTGTCT80.21356113187399892No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAAAATAAAAGCAGAGAC70.18686599038974908No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC70.18686599038974908No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC70.18686599038974908No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC70.18686599038974908No Hit
AGCCTAGACATCCACTGCTGCGTGGGCTCGGAGATGTGGTAATGATCCTT70.18686599038974908No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGCAGAGAC60.16017084890549918No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC60.16017084890549918No Hit
AGCCTAGACATCCACTGCTGCTCCGTGGTAATGAACCTTCCGCTGTCTCT60.16017084890549918No Hit
AGCCTAGACATCCACTGCTGCCGACGTGTGGTAATGATCCTTCCGCTGTC60.16017084890549918No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC60.16017084890549918No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGGCTCGGAGTGGTAATGATCCT60.16017084890549918No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAATTAGAGAC60.16017084890549918No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGCAGAGAC50.13347570742124934No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC50.13347570742124934No Hit
AGCCTAGATATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC50.13347570742124934No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC50.13347570742124934No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGCAGAGAC50.13347570742124934No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATCTAGTAAAAGCAGGGACTT40.10678056593699946No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC40.10678056593699946No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC40.10678056593699946No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT40.10678056593699946No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAAAGAC40.10678056593699946No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)