FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OODve771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OODve771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14787
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC389726.354230066950702No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT264417.880570771623724No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT240616.27104889429905No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT153410.373977142084263No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT3852.6036383309663895No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC3652.4683843916954085No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTCAAAAGCAGAGACT2041.379590180564009No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC1390.9400148779333197No Hit
AGCCTAGACATCCACTGCTGTTCTTAGTGGTAATGATCCTTCCGCTGTCT1100.7438966659903969No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC940.635693514573612No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC790.534253060120376No Hit
AGCCTAGACATCCACTGCTGACGAGTGGTAATGATCCTTCCGCTGTCTCT780.527490363156827No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTTAAAAGCAGAGACT500.3381348481774532No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT440.2975586663961588No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGTAGAGAC420.2840332724690607No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC270.18259281801582472No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC250.1690674240887266No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGAACT230.15554203016162846No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC220.1487793331980794No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC210.14201663623453034No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAGCT200.13525393927098125No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC180.12172854534388314No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT180.12172854534388314No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC180.12172854534388314No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC180.12172854534388314No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAAGAACT180.12172854534388314No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCAAGTTAAAAGCAAGAACT170.11496584838033408No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC160.10820315141678502No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC150.10144045445323595No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACC150.10144045445323595No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC150.10144045445323595No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCGGAGACT150.10144045445323595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)