FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OODve769.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OODve769.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10764
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT588854.700854700854705No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT147813.73095503530286No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT6245.797101449275362No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT4223.9204756596060943No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCAATAAAAGCAAGGACTTT1521.4121144555927163No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTTAAAGCAAGAACT1481.3749535488665925No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT1441.3377926421404682No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT580.5388331475287997No Hit
AGCCTAGACATCCACTGCTGCCGACGATGTGGTAATGATCCTTCCGCTGT490.4552211073950204No Hit
AGCCTAGACATCCACTGCTGCCGAGGTGGTAATGATCCTTCCGCTGTCTC450.4180602006688963No Hit
AGCCTAGACATCCACTGCTGCCGACGACTGTGGTAATGATCCTTCCGCTG450.4180602006688963No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTAAGAGCAAGGACTT340.315867707172055No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTC330.30657748049052397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAGAGCAAGGACTT300.2787068004459309No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTAAGAGCAAGGACTT280.2601263470828688No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGGGCAAGGACTT260.24154589371980675No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGATTGAAAGCAAAGACT250.23225566703827574No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTAAGAGCAAGGACTT220.20438498699368265No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAATAAGAGCAAGGACTT220.20438498699368265No Hit
AGCCTAGACATCCACTGCTGACGGTGGTAATGATCCTTCCGCTGTCTCTT190.17651430694908954No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAGGGACTT170.1579338535860275No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTAAGAGCAAGGACTT160.14864362690449648No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACCT160.14864362690449648No Hit
AGCCTAGACATCCACTGCTGTCTCTTATACACATCTGACGCTGCCGACGA150.13935340022296544No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTAAGAGCAAGGACTT150.13935340022296544No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTAAGAGCAAGGACTT150.13935340022296544No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAGGAGCAAGGACTT150.13935340022296544No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC140.1300631735414344No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCGAGGACTT130.12077294685990338No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGTAAGAGCAAGGACTT130.12077294685990338No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC130.12077294685990338No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGTAAGAGCAAGGACTT130.12077294685990338No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGGCTT120.11148272017837235No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAGTTAAAAGCAGAGACT110.10219249349684133No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGTAAGAGCAAGGACTT110.10219249349684133No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGTAAGAGCAAGGACTT110.10219249349684133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)