FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi754.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi754.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14276
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC575140.284393387503506No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT212014.850098066685346No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT10667.467077612776688No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATGGATTAAAACCAAAAACTT9146.402353600448304No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT8465.926029700196133No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC3802.6618100308209582No Hit
AGCCTAGACATCCACTGCTGCTCGTGGTAATGATCCTTCCGCTGTCTCTT2361.653124124404595No Hit
AGCCTAGACATCCACTGCTGTCGTGGTAATGATCCTTCCGCTGTCTCTTA2041.4289717007565146No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTATCTAGAATAAAAGTAGAGACT830.5813953488372093No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT410.2871952927991034No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGTAGAGAC380.26618100308209586No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC340.2381619501260857No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC330.23115718688708323No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC300.21014289717007564No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC300.21014289717007564No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTTTTGATTAAAACCAAAGACTT280.1961333706920706No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC280.1961333706920706No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGATAAAAGCAGAGACT260.18212384421406558No Hit
AGCCTAGACATCCACTGCTGTCTCTTATACACATCTGACGCTGCCGACGA250.17511908097506304No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC240.1681143177360605No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC240.1681143177360605No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC210.14710002801905295No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGTAGAGAC210.14710002801905295No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC210.14710002801905295No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC210.14710002801905295No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC190.13309050154104793No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC190.13309050154104793No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC180.1260857383020454No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC170.11908097506304285No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGTAGAGAC170.11908097506304285No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC160.11207621182404036No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACAATAAAAGTAGAGAC160.11207621182404036No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTTAGGATTAAAACCAAGACTTT150.10507144858503782No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTTAGAATAAAAGTAGAGAC150.10507144858503782No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)