FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi749.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi749.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2234
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT37616.83079677708147No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT33715.085049239033124No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC2209.847806624888094No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC1436.401074306177261No Hit
AGCCTAGACATCCACTGCTGCGGAGATGTGGTAATGATCCTTCCGCTGTC893.9838854073410923No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT853.8048343777976723No Hit
AGCCTAGACATCCACTGCTGGGTCTCGTGGTAATGATCCTTCCGCTGTCT833.715308863025962No Hit
AGCCTAGACATCCACTGCTGGGAGATGTGGTAATGATCCTTCCGCTGTCT823.670546105640107No Hit
AGCCTAGACATCCACTGCTGAGATGTGGTAATGATCCTTCCGCTGTCTCT823.670546105640107No Hit
AGCCTAGACATCCACTGCTGGATGTGGTAATGATCCTTCCGCTGTCTCTT783.491495076096687No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTGAAAGCAGAGACT602.685765443151298No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT401.7905102954341987No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC271.2085944494180842No Hit
AGCCTAGACATCCACTGCTGTGTGTATAAGAGACAGTGGTAATGATCCTT261.1638316920322291No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT190.8504923903312444No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT90.40286481647269473No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC80.35810205908683973No Hit
AGCCTAGACATCCACTGCTGCCGACGGTGGTAATGATCCTTCCGCTGTCT80.35810205908683973No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC70.3133393017009848No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTACGCTGTCTCTTATAC70.3133393017009848No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTGAAAGCAGAGGCT50.22381378692927484No Hit
AGCCTAGACATCCACTGCTGGGTGTGGTAATGATCCTTCCGCTGTCTCTT50.22381378692927484No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTGAAAGCAGAGACT50.22381378692927484No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAGAGCAAGAATT50.22381378692927484No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTTCGCTGTCTCTTATAC50.22381378692927484No Hit
AGCCTAGACATCCACTGCTGTGGTAATGAACCTTCCGCTGTCTCTTATAC40.17905102954341987No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAAATTT40.17905102954341987No Hit
CGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT40.17905102954341987No Hit
AGCCTAGACATCCACTGCTGGGTCTCGTTGTAATGATCCTTCCGCTGTCT30.13428827215756492No Hit
AGCCTAGACATCCACTGCTGGATGTGGTAATGATACTTCCGCTGTCTCTT30.13428827215756492No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT30.13428827215756492No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAT30.13428827215756492No Hit
AGCCTAGACATCCACTGCTGGGAGATGTGGTAATGATCCTTCCGCTGTCC30.13428827215756492No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGTTAAAAGCAAGGACT30.13428827215756492No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAACGATCCTTCCGCTGTC30.13428827215756492No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT30.13428827215756492No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTTAAAAGCAAGGACT30.13428827215756492No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATGTAATTAAAAGCAAGAATT30.13428827215756492No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAGGCAAGAATT30.13428827215756492No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTACCTAGTTGAAAGCAGAGACT30.13428827215756492No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTTAAAAGCAAGGACT30.13428827215756492No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT30.13428827215756492No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTACTTAAAAGCAAGAATT30.13428827215756492No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)