FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC84563.29588014981273No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT735.468164794007491No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC624.644194756554307No Hit
AGCCTAGACATCCACTGCTGCCGTGGTAATGATCCTTCCGCTGTCTCTTA402.9962546816479403No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT332.4719101123595504No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC141.0486891385767791No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC80.599250936329588No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGTAGAGAC70.5243445692883896No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT70.5243445692883896No Hit
AGCCTAGACATCCACTGCTGCCACGGTGGTAATGATCCTTCCGCTGTCTC60.44943820224719105No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC60.44943820224719105No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC60.44943820224719105No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC40.299625468164794No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC40.299625468164794No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC40.299625468164794No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAGAGCAAGGACT30.22471910112359553No Hit
AGCCTAGACATCCATTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC30.22471910112359553No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGTAGAGAC30.22471910112359553No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC30.22471910112359553No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC30.22471910112359553No Hit
AGCCTAGACATCCACTGCTGAAAGTTATTATCTAGAATAAAAGTAGAGAC30.22471910112359553No Hit
AGCCTATACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC30.22471910112359553No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC30.22471910112359553No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC30.22471910112359553No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGCC30.22471910112359553No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTTGAGAC30.22471910112359553No Hit
AGCCTAGACATCCACTGCTGAAAGTTTTTATCTTATTTTATTGCGCAAGT30.22471910112359553No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC20.149812734082397No Hit
CGGCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGT20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATGCAGAATAAAAGTAGAGAC20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAACGCAAGGACTT20.149812734082397No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC20.149812734082397No Hit
GAGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGA20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACAATAAAAGCAGAGAC20.149812734082397No Hit
CGGCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGT20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCAAGAATAAAAGTAGAGAC20.149812734082397No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGGTCCTTCCGCTGTCTC20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAATAGCAAGGGCT20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTTAAAAGCAAGGACT20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAAGGACT20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAAAATAAAAGTAGAGAC20.149812734082397No Hit
AGCCTAGACATCCACTGCTGGCCTCGTGGTAATGATCCTTCCGCTGTCTC20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATATAGAATAAAAGTAGAGAC20.149812734082397No Hit
AGCCTAGAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGACT20.149812734082397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC20.149812734082397No Hit
AGCCAAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC20.149812734082397No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC20.149812734082397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)