FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi747.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi747.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16767
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT718042.82221029402994No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT169610.115107055525735No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT15899.476948768414147No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC14058.379555078427863No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC7034.192759587284547No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT3962.3617820719269997No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC2441.4552394584600705No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGGCTCGGAGATGTGGTAATGATC1170.6977992485238862No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACC590.351881672332558No Hit
AGCCTAGACATCCACTGCTGTCTGTGGTAATGATCCTTCCGCTGTCTCTT590.351881672332558No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT540.322061191626409No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT510.3041689032027196No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTTAAAAGCAAGGACT510.3041689032027196No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGGACT440.2624202302141111No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAAGGACT380.22663565336673228No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTT370.22067155722550247No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTTAAAAGCAAGGACT360.2147074610842727No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAAGGACT340.20277926880181307No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTTAAAAGCAAGGACT310.1848869803781237No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTTAAAAGCAAGGACT310.1848869803781237No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAATTAAAAGCAAGGACT290.17295878809566412No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT290.17295878809566412No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGAGGACT280.1669946919544343No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAGAAGCAAGGACT250.14910240353074491No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTTAAAAGCAAGGACT250.14910240353074491No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGTTAAAAGCAAGGACT250.14910240353074491No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT250.14910240353074491No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATGGATTAAAACCAAAAACTT230.1371742112482853No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGTTAAAAGCAAGGACT220.13121011510705555No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGAGACTT210.12524601896582574No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTTAAAAGCAAGGACT200.11928192282459593No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAGGCAGGGACTT190.11331782668336614No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT190.11331782668336614No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAGAGCAAGGACT190.11331782668336614No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGCAGAGAC180.10735373054213634No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCTAAAAGCAAGGACT180.10735373054213634No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGGCT170.10138963440090654No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)