FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi744.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi744.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4576
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT162135.42395104895105No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT101522.180944055944053No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT3437.495629370629371No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC1372.9938811188811187No Hit
AGCCTAGACATCCACTGCTGACGCTGCCGACGAGTGGTAATGATCCTTCC801.7482517482517483No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT701.5297202797202798No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGGCTCGGAGATGTGGTAATGAT691.507867132867133No Hit
AGCCTAGACATCCACTGCTGTAGTGTGGTAATGATCCTTCCGCTGTCTCT651.4204545454545454No Hit
AGCCTAGACATCCACTGCTGCCGACGTGGTAATGATCCTTCCGCTGTCTC521.1363636363636365No Hit
AGCCTAGACATCCACTGCTGCTCGTGGTAATGATCCTTCCGCTGTCTCTT380.8304195804195804No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT240.5244755244755245No Hit
AGCCTAGACATCCACTGCTGCAGATGTGTATAAGAGACAGTGGTAATGAT230.5026223776223776No Hit
AGCCTAGACATCCACTGCTGTAAGTTGCTATCTAGTTAAAAGCAGAGACT220.4807692307692308No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC200.4370629370629371No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGAACT170.3715034965034965No Hit
AGCCTAGACATCCACTGCTGAGAGTTGCTATCTAGTTAAAAGCAGAGACT170.3715034965034965No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTACCTAGTTAAAAGCAGAGACT150.3277972027972028No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAGAGCAGAGACT150.3277972027972028No Hit
AGCCTAGACATCCACTGCTGTGGGCTCGGAGATGTGGTAATGATCCTTCC140.30594405594405594No Hit
AGCCTAGACATCCACTGCTGAAAGTCGCTATCTAGTTAAAAGCAGAGACT130.2840909090909091No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT110.2403846153846154No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT110.2403846153846154No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTTAAAAGCAGAGACT100.21853146853146854No Hit
AGCCTAGACATCCACTGCTGGTGGTAATGATCCTTCCGCTGTCTCTTATA100.21853146853146854No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGGCT90.1966783216783217No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAGGCAGAGACT90.1966783216783217No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAGAAGCAGAGACT80.17482517482517482No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGGGACT80.17482517482517482No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTTAAAAGCAAGAACT80.17482517482517482No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACC80.17482517482517482No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCGGAGACT70.15297202797202797No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCCATCTAGTTAAAAGCAGAGACT70.15297202797202797No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCCAGTTAAAAGCAGAGACT70.15297202797202797No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTTAAAAGCAGAGACT70.15297202797202797No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATCTAGTTAAAAGCAGAGACT70.15297202797202797No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTGGTTAAAAGCAGAGACT70.15297202797202797No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGTTAAAAGCAAGAACT60.13111888111888112No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGAACT60.13111888111888112No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAAGACT60.13111888111888112No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAAGAACT60.13111888111888112No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTGTCTAGTTAAAAGCAGAGACT60.13111888111888112No Hit
AGCCTAGACATCCACTGCTGTCCCGTGGTAATGATCCTTCCGCTGTCTCT60.13111888111888112No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGCTAAAAGCAGAGACT60.13111888111888112No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCATGAACT60.13111888111888112No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGTTAAAAGCAAGAACT50.10926573426573427No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTACTTAAAAGCAGAGACT50.10926573426573427No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGAGAACT50.10926573426573427No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTTAAAAGCAAGAACT50.10926573426573427No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAAGAACT50.10926573426573427No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTATTTAAAAGCAGAGACT50.10926573426573427No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAATTAAAAGCAGAGACT50.10926573426573427No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)