FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi740.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi740.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC107974.61964038727524No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT765.255878284923928No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT231.590594744121715No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC221.5214384508990317No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGTAGAGAC100.6915629322268326No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC100.6915629322268326No Hit
AGCCTAGACATCCACTGCTGACGACGTGGTAATGATCCTTCCGCTGTCTC90.6224066390041494No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC90.6224066390041494No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC80.5532503457814661No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC70.48409405255878285No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT70.48409405255878285No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC60.4149377593360996No Hit
AGCCTAGACATCCACTGCTGTCTGTGGTAATGATCCTTCCGCTGTCTCTT60.4149377593360996No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAAAATAAAAGTAGAGAC60.4149377593360996No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC50.3457814661134163No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC50.3457814661134163No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC40.2766251728907331No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC40.2766251728907331No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC30.2074688796680498No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC30.2074688796680498No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC30.2074688796680498No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC30.2074688796680498No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC30.2074688796680498No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC30.2074688796680498No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATGGATTAAAACCAAAAACTT20.13831258644536654No Hit
AGCCTAGACATCCACTTCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC20.13831258644536654No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTGGTTAAAAGCAGAGACT20.13831258644536654No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTGGAGAC20.13831258644536654No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT20.13831258644536654No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATGTAGAATAAAAGTAGAGAC20.13831258644536654No Hit
AGCCTAGACACCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC20.13831258644536654No Hit
AGCCCAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC20.13831258644536654No Hit
AGCCTAGACATCCACTGCTGAAAATTGTTATCTAGAATAAAAGTAGAGAC20.13831258644536654No Hit
ACTCCATGGAGTCCACCGGAGACCTTGTAGCTGTTCTGGTTGAACTTGCC20.13831258644536654No Hit
AGCCTAGACATCCACTGCTGAGTGGTAATGATCCTTCCGCTGTCTCTTAT20.13831258644536654No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGTAGAGAC20.13831258644536654No Hit
AGCCTAGACTCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGACT20.13831258644536654No Hit
AGCCTAAACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC20.13831258644536654No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACAATAAAAGTAGAGAC20.13831258644536654No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)