FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi737.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi737.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6010
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT208734.72545757071547No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC126621.064891846921796No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT84914.126455906821963No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT3355.574043261231281No Hit
AGCCTAGACATCCACTGCTGATGTGGTAATGATCCTTCCGCTGTCTCTTA781.29783693843594No Hit
AGCCTAGACATCCACTGCTGACGAGTGGTAATGATCCTTCCGCTGTCTCT741.2312811980033278No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT580.9650582362728786No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC520.8652246256239602No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT520.8652246256239602No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC460.7653910149750416No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT460.7653910149750416No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT350.5823627287853578No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGGACT160.26622296173044924No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTTAAAAGCAAGGACT140.23294509151414308No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC110.18302828618968386No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAAGGACT110.18302828618968386No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTTAAAAGCAAGGACT100.16638935108153077No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC100.16638935108153077No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC100.16638935108153077No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCTAAAAGCAAGGACT90.1497504159733777No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTCAAAAGCAGAGACT90.1497504159733777No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT90.1497504159733777No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGGCT80.13311148086522462No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC80.13311148086522462No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAAGGACT70.11647254575707154No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC70.11647254575707154No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCCATCTAGTTAAAAGCAGAGACT70.11647254575707154No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTACCTAGTTAAAAGCAGAGACT70.11647254575707154No Hit
AGCCTAGACATCCACTGCTGCCGACGGTGGTAATGATCCTTCCGCTGTCT70.11647254575707154No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC70.11647254575707154No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAGAAGCAAGGACT70.11647254575707154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)