FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi733.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi733.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10664
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC898084.2085521380345No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGTAGAGAC830.7783195798949738No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC550.5157539384846211No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC440.41260315078769694No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC380.3563390847711928No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAAAATAAAAGTAGAGAC350.32820705176294074No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC330.3094523630907727No Hit
AGCCTAGACATCCACTGCTGAAAGTTGATATCTAGAATAAAAGTAGAGAC320.30007501875468867No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC320.30007501875468867No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC290.2719429857464366No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC280.2625656414103526No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGTAGAGAC270.25318829707426854No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC270.25318829707426854No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC260.24381095273818457No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC260.24381095273818457No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGTAGAGAC260.24381095273818457No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC250.23443360840210054No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC240.22505626406601648No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC230.2156789197299325No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC210.19692423105776444No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC200.1875468867216804No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC200.1875468867216804No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACAATAAAAGTAGAGAC200.1875468867216804No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC190.1781695423855964No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGTAGAGAC190.1781695423855964No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC160.15003750937734434No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAT160.15003750937734434No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC150.1406601650412603No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC150.1406601650412603No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC150.1406601650412603No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAAAC140.1312828207051763No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTGGAGAC140.1312828207051763No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAACAAAAGTAGAGAC140.1312828207051763No Hit
AGCCTAGACATCCACTGCTGACAGTTGTTATCTAGAATAAAAGTAGAGAC130.12190547636909228No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGTAGAGAC130.12190547636909228No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC130.12190547636909228No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC120.11252813203300824No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCGAGAATAAAAGTAGAGAC110.10315078769692423No Hit
AGCCTAGACACCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC110.10315078769692423No Hit
AGCCTAGACATCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGACT110.10315078769692423No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)