FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi725.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi725.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21788
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT824437.83734165595741No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT585326.863411051955204No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGTAGAGACT246511.3135671011566No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT3411.5650816963466128No Hit
AGCCTAGACATCCACTGCTGTTCGTGGTAATGATCCTTCCGCTGTCTCTT2491.1428309161006058No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC2311.060216633008996No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC1550.7114007710666422No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC1380.6333761703690105No Hit
AGCCTAGACATCCACTGCTGGCGTCAGATGTGGTAATGATCCTTCCGCTG1230.5645309344593354No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT750.34422617954837526No Hit
AGCCTAGACATCCACTGCTGAGAGTTGCTATCTAGTTAAAAGCAGAGACT750.34422617954837526No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACCT680.3120984027905269No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCGAGGACTT560.25702221406278686No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGGGACT520.23866348448687352No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT490.2248944373049385No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTC440.2019460253350468No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTAAAAGCAAGGACTT430.19735634294106846No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGTAGAGACT430.19735634294106846No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAGAAGCAAGGACTT410.1881769781531118No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTT400.18358729575913346No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAGGCAAGGACTT400.18358729575913346No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATCTAGTTAAAAGCAGAGACT390.17899761336515513No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACC390.17899761336515513No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAGGCAGAGACT380.1744079309711768No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTAAAAGCAAGGACTT370.16981824857719846No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAATTAAAAGCAGAGACT370.16981824857719846No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTAAAAGCAAGGACTT350.1606388837892418No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTAAAAGCAAGGACTT320.14686983660730676No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT300.1376904718193501No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTAAAAGCAAGGACTT290.13310078942537176No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTTAAAAGCAGAGACT290.13310078942537176No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAATAAAAGCAAGGACTT280.12851110703139343No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGTAAAAGCAAGGACTT270.1239214246374151No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCGGAGACT260.11933174224343676No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGTAAAAGCAAGGACTT250.11474205984945841No Hit
AGCCTAGACATCCACTGCTGAAAGCTGCTATCTAGTTAAAAGCAGAGACT250.11474205984945841No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGTAAAAGCAAGGACTT240.11015237745548008No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAGAGCAGAGACT240.11015237745548008No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGTAAAAGCAAGGACTT230.10556269506150175No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAGAAGCAGAGACT230.10556269506150175No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTTAAAAGCAGAGACT230.10556269506150175No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGGCTT230.10556269506150175No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTAAAAGCAAGGACTT220.1009730126675234No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTATTTAAAAGCAGAGACT220.1009730126675234No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)