FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi717.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi717.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3014
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT51717.153284671532848No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC46115.295288652952888No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT35911.911081619110817No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC32510.783012607830127No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGCTCGGAGATGTGGTAATGATC2337.730590577305906No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT2046.768414067684141No Hit
AGCCTAGACATCCACTGCTGCCGACGATGTGGTAATGATCCTTCCGCTGT1504.97677504976775No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC1344.445919044459191No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT822.72063702720637No Hit
AGCCTAGACATCCACTGCTGCCGACGGTGTGGTAATGATCCTTCCGCTGT70.232249502322495No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAGGCAAGAATT70.232249502322495No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT70.232249502322495No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC60.19907100199071004No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC60.19907100199071004No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCTAAAAGCAAGAACT50.16589250165892502No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAGAGCAAGAATT50.16589250165892502No Hit
AGCCTAGACATCCACTGCTGCCGACGGTGGTAATGATCCTTCCGCTGTCT50.16589250165892502No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC50.16589250165892502No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAGTTAAAAGCAAGAATT50.16589250165892502No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAACT50.16589250165892502No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAGGCAAGGACTTT50.16589250165892502No Hit
AGCCTAGACATCCACTGCTGGTTTCGTGGTAATGATCCTTCCGCTGTCTC40.13271400132714No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC40.13271400132714No Hit
AGCCTAGACATCCACTGCTGGTCTCGTTGTAATGATCCTTCCGCTGTCTC40.13271400132714No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT40.13271400132714No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAGTT40.13271400132714No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACC40.13271400132714No Hit
AGCCTAGACATCCACTGCTGAGAGTTGCTATTTAATTAAAAGCAAGAATT40.13271400132714No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)