FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi716.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi716.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGAGAACT28819.57851801495581No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT23716.11148878314072No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC17611.964649898028552No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTCAAAGCAAGAACT15110.265125764785859No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT966.5261726716519375No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT493.331067301155676No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGATTGAAAGCAAAGACT412.7872195785180147No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAAGACT302.0394289598912305No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT291.9714479945615229No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT261.7675050985723997No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT221.495581237253569No Hit
AGCCTAGACATCCACTGCTGTGTGGTAATGATCCTTCCGCTGTCTCTTAT181.2236573759347382No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT151.0197144799456153No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAATT151.0197144799456153No Hit
AGCCTAGACATCCACTGCTGTCGGCGCGCAGTGGTAATGATCCTTCCGCT90.6118286879673691No Hit
AGCCTAGACATCCACTGCTGTGGTACTGATCCTTCCGCTGTCTCTTATAC60.4078857919782461No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTACGCTGTCTCTTATAC60.4078857919782461No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGGTTAAAACCAAAGACTT60.4078857919782461No Hit
AGCCTAGACATCCACTGCTGTCTCTGTGGTAATGATCCTTCCGCTGTCTC60.4078857919782461No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCGAGAACT40.27192386131883073No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTTCGCTGTCTCTTATAC40.27192386131883073No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTGCGCTGTCTCTTATAC40.27192386131883073No Hit
AGCCTAGACATCCACTGCTGTAAGTTGTTATTGATTAAAACCAAAGACTT40.27192386131883073No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGGGAACT30.20394289598912305No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTCAAAGCAAGAACT30.20394289598912305No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTT30.20394289598912305No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTTAAAAGCGAGAACT30.20394289598912305No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC20.13596193065941536No Hit
AGTCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGAGAACT20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCTAAAAGCGAGAACT20.13596193065941536No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTTTATACA20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGTAAAAGCAAGGACTT20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAAGACT20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGACTAAAACCAAAGACTT20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAATTCAAAGCAAGAACT20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTTAAAAGCGAGAACT20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCAGCTGTCTCTTATAC20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTCAAAGCAAGAGCT20.13596193065941536No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATTGATTAAAACCAAAGACTT20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACCT20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCGAGAACT20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGAGGACT20.13596193065941536No Hit
AGCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATACA20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT20.13596193065941536No Hit
AGCCTAGACTCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTTT20.13596193065941536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTAAAAGCAAGGACTT20.13596193065941536No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGGCTT20.13596193065941536No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)