FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi705.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi705.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3420
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT90226.374269005847957No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT83024.269005847953213No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT71320.84795321637427No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT1875.4678362573099415No Hit
AGCCTAGACATCCACTGCTGCCGACGATGTGGTAATGATCCTTCCGCTGT1374.005847953216374No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTATTGAATTTAATAACAACCGCT160.46783625730994155No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT150.43859649122807015No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTACCTAGTTAAAAGCAGAGACT140.40935672514619886No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACC140.40935672514619886No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT120.3508771929824561No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAGAGCAGAGACT120.3508771929824561No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT110.32163742690058483No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTC80.23391812865497078No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAGGCAGAGACT80.23391812865497078No Hit
AGCCTAGACATCCACTGCTGAGAGTTGCTATCTAGTTAAAAGCAGAGACT70.20467836257309943No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAGTTAAAAGCAAGAATT60.17543859649122806No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGGCT60.17543859649122806No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATTTAATTAAAAGCAAGAATT60.17543859649122806No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCGGAGACT50.14619883040935672No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACCT50.14619883040935672No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGTAAAAGCAAGGACTT50.14619883040935672No Hit
AGCCTAGACATCCACTGCTGACAGTTGTTATCTAGTAAAAGCAAGGACTT50.14619883040935672No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACTAAAAGCAAGGACTT50.14619883040935672No Hit
CGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT50.14619883040935672No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT50.14619883040935672No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTAAAAGCAAGGACTT40.11695906432748539No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCGAGTAAAAGCAAGGACTT40.11695906432748539No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTAAAAGCAAGGACTT40.11695906432748539No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAGAAGCAGAGACT40.11695906432748539No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACC40.11695906432748539No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATTTAATTAAAAGCAAGAATT40.11695906432748539No Hit
AGCCTAGACATCCATTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT40.11695906432748539No Hit
AGCCTGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATTT40.11695906432748539No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGGGACT40.11695906432748539No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAATTAAAAGCAGAGACT40.11695906432748539No Hit
AGCCTAGACATCCACTGCTGAAAGTTTCTATCTAGTTAAAAGCAGAGACT40.11695906432748539No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)