FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi700.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi700.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2996
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC186462.21628838451269No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT54618.22429906542056No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT1043.4712950600801067No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGTAGAGAC160.5340453938584779No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC130.43391188251001334No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC110.36715620827770357No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC100.33377837116154874No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC90.30040053404539385No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC90.30040053404539385No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC80.26702269692923897No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC80.26702269692923897No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGTAGAGAC80.26702269692923897No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTT80.26702269692923897No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC80.26702269692923897No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC70.23364485981308408No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC70.23364485981308408No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC60.20026702269692925No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAAAATAAAAGTAGAGAC60.20026702269692925No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAATAAAAGCAGGGACTT60.20026702269692925No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC60.20026702269692925No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGTAGAGAC60.20026702269692925No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTATTAAAAGCAGGGACTT50.16688918558077437No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC50.16688918558077437No Hit
AGCCTAGACATCCACTGCCGAAAGTTGTTATCTAGAATAAAAGTAGAGAC50.16688918558077437No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTGAAAGCAGGGACTT50.16688918558077437No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC50.16688918558077437No Hit
AGCCTAGACATCCATTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC40.13351134846461948No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGTAGAGAC40.13351134846461948No Hit
AGCCTAGACATCCAATGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC40.13351134846461948No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAACAAAAGTAGAGAC40.13351134846461948No Hit
AGCCTAGACATCCACTGCTTAAAGTTGTTATCTAGAATAAAAGTAGAGAC40.13351134846461948No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC40.13351134846461948No Hit
AGCCTAGACATCCACTGCTGAAAGTTTCTATCTAGTAAAAGCAGGGACTT40.13351134846461948No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCATGGACTT40.13351134846461948No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC40.13351134846461948No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTGGAGAC40.13351134846461948No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTAACTAGTAAAAGCAGGGACTT40.13351134846461948No Hit
AGCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGACT40.13351134846461948No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGGCTT40.13351134846461948No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACAATAAAAGTAGAGAC40.13351134846461948No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC30.10013351134846463No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC30.10013351134846463No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT30.10013351134846463No Hit
AGCCTAGACATCCACTGCTGAGAGTTGCTATCTAGTAAAAGCAGGGACTT30.10013351134846463No Hit
AGCCTATACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC30.10013351134846463No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAAAC30.10013351134846463No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC30.10013351134846463No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT30.10013351134846463No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTAAAAGCAGGGACTT30.10013351134846463No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGAATT30.10013351134846463No Hit
GAGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACT30.10013351134846463No Hit
AGCCTAGACATCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGACT30.10013351134846463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)