FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi694.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi694.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10758
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC812675.53448596393382No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT3633.374233128834356No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT1961.8218999814091839No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT1231.143335192414947No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGTAGAGAC670.6227923405837517No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC510.4740658114891244No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC450.4182933630786392No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC440.408997955010225No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC420.39040713887339656No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC420.39040713887339656No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGTAGAGAC410.38111173080498234No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC350.3253392823944971No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC350.3253392823944971No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC340.3160438743260829No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC340.3160438743260829No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC340.3160438743260829No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAAAATAAAAGTAGAGAC320.2974530581892545No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC320.2974530581892545No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC310.2881576501208403No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC290.26956683398401193No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC290.26956683398401193No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC260.2416806097787693No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC250.23238520171035507No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT240.2230897936419409No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAAAC220.2044989775051125No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTGGAGAC220.2044989775051125No Hit
AGCCTAGACATCCACTGCTGGGTGGTAATGATCCTTCCGCTGTCTCTTAT220.2044989775051125No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACAATAAAAGTAGAGAC210.19520356943669828No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC210.19520356943669828No Hit
AGCCTAGACATCCACTGCTGAAAGTTACTATCTAGTTAAAAGCAGAGACT200.18590816136828406No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAAGACTT200.18590816136828406No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGTAGAGAC200.18590816136828406No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC190.17661275329986986No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC170.15802193716304144No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC170.15802193716304144No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGTAGAGAC150.13943112102621305No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAACAAAAGTAGAGAC150.13943112102621305No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGTAGAGAC140.13013571295779885No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCAAGAATAAAAGTAGAGAC120.11154489682097045No Hit
AGCCCAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC120.11154489682097045No Hit
AGCCTAGACATCCATTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC110.10224948875255625No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC110.10224948875255625No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC110.10224948875255625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)