FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi693.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi693.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12853
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC1055382.10534505562903No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCAAGTAAAAGTGAGAACTT1000.7780284758422159No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGTAGAGAC670.5212790788142846No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC660.5134987940558624No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC590.45903680074690734No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC560.43569594647164084No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC550.42791566171321865No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC540.4201353769547966No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC510.3967945226795301No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT450.3501128141289971No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAAAATAAAAGTAGAGAC440.34233252937057496No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC440.34233252937057496No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC370.28787053606161983No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC370.28787053606161983No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC370.28787053606161983No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC360.2800902513031977No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC360.2800902513031977No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC340.2645296817863534No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC320.24896911226950907No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC320.24896911226950907No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTGGAGAC290.22562825799424258No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC290.22562825799424258No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC290.22562825799424258No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC280.21784797323582042No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGTAGAGAC280.21784797323582042No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGTAGAGAC280.21784797323582042No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC270.2100676884773983No Hit
AGCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGACT210.16338597992686532No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGTAGAGAC200.15560569516844316No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGTAGAGAC190.147825410410021No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACAATAAAAGTAGAGAC190.147825410410021No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC190.147825410410021No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT170.1322648408931767No Hit
AGCCTAGACATCCACTGCTGTAAGTTGTTATCTAGAATAAAAGTAGAGAC170.1322648408931767No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAAAC150.11670427137633237No Hit
AGCCTAGACTCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGACT140.10892398661791021No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAACAAAAGTAGAGAC140.10892398661791021No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC140.10892398661791021No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC130.10114370185948807No Hit
AGCCTAGACATCCACCGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC130.10114370185948807No Hit
AGCCTAGACATCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGACT130.10114370185948807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)