FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi691.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi691.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5346
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT190735.67153011597456No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT67812.682379349046016No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTATCTAGTTAAGAGTAAAAACTT3837.164234942012719No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC2664.975682753460531No Hit
AGCCTAGACATCCACTGCTGGAGATGTGGTAATGATCCTTCCGCTGTCTC1973.6849981294425738No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC1913.5727646838757945No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC1542.880658436213992No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT1152.1511410400299287No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT781.4590347923681257No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC751.4029180695847363No Hit
AGCCTAGACATCCACTGCTGAGTGGTAATGATCCTTCCGCTGTCTCTTAT691.2906846240179575No Hit
AGCCTAGACATCCACTGCTGTCTCTTATACACATCTGACGCTGCCGACGA520.9726898615787506No Hit
AGCCTAGACATCCACTGCTGTGTATAAGAGACAGTGGTAATGATCCTTCC490.9165731387953611No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT310.5798728020950242No Hit
AGCCTAGACATCCACTGCTGGGTCTCGTGGGCTCGGAGATGTGGTAATGA260.4863449307893753No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT230.4302282080059858No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATCTAGTAAAAGCAGGGACTT160.29928918817807704No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTAAAAGCAGGGACTT120.22446689113355783No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTAAAAGCAGGGACTT120.22446689113355783No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAGAAGCAGGGACTT110.205761316872428No Hit
AGCCTAGACATCCACTGCTGAAAGCTGCTATCTAGTAAAAGCAGGGACTT100.18705574261129815No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTC100.18705574261129815No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTC100.18705574261129815No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCATGGACTT100.18705574261129815No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGAGACTT100.18705574261129815No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAACAGGGACTT80.14964459408903852No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGGGACT80.14964459408903852No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCCAGTAAAAGCAGGGACTT80.14964459408903852No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTATTAAAAGCAGGGACTT70.1309390198279087No Hit
AGCCTAGACATCCACTGCTGAAAGTCGCTATCTAGTAAAAGCAGGGACTT70.1309390198279087No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTATCTAGTTAAGAGTAAAAACTT70.1309390198279087No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTT70.1309390198279087No Hit
ACCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTTT60.11223344556677892No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCCATCTAGTAAAAGCAGGGACTT60.11223344556677892No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTGTCTAGTAAAAGCAGGGACTT60.11223344556677892No Hit
CGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT60.11223344556677892No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAGGCAGAGACT60.11223344556677892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)