FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi686.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi686.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17599
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC1474683.78885163929769No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGTAGAGAC1380.7841354622421729No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC770.43752485936700947No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC730.414796295244048No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC650.36933916699812486No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC630.3579748849366441No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC620.3522927439059037No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC590.3352463208136826No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAAAATAAAAGTAGAGAC570.32388203875220184No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC550.31251775669072107No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC520.2954713335984999No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC500.28410705153701915No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC500.28410705153701915No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC500.28410705153701915No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC490.27842491050627877No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC470.267060628444798No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC470.267060628444798No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACAATAAAAGTAGAGAC440.25001420535257685No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC430.24433206432183646No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC430.24433206432183646No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGTAGAGAC430.24433206432183646No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTGGAGAC410.2329677822603557No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGTAGAGAC390.22160350019887493No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC270.15341780782999034No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAACAAAAGTAGAGAC270.15341780782999034No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGAATAAAAGTAGAGAC270.15341780782999034No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC250.14205352576850958No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC220.12500710267628842No Hit
AGCCTAGACATCCACTGCTGACAGTTGTTATCTAGAATAAAAGTAGAGAC200.11364282061480765No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGTAGAGAC200.11364282061480765No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC200.11364282061480765No Hit
AGCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGACT190.10796067958406727No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAT190.10796067958406727No Hit
AGCCTATACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC180.1022785385533269No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATATAGAATAAAAGTAGAGAC180.1022785385533269No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)