FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi682.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi682.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21232
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT648930.562358703843255No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT508823.9638281838734No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC508423.94498869630746No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAGTAAAAGCAGGGACTT1240.5840241145440844No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT1150.5416352675207234No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC790.3720798794272796No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTT730.34382064807837226No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGAGACTT590.2778824415975885No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT570.26846269781461946No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC460.21665410700828935No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTTAAAAGCAGAGACT460.21665410700828935No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTTAAAAGCAGAGACT450.21194423511680482No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGGACT410.19310474755086662No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTTAAAAGCAGAGACT410.19310474755086662No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC380.17897513187641298No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAGAGACT370.17426525998492842No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACCT360.16955538809344384No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAAACT340.16013564431047475No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTC340.16013564431047475No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACC340.16013564431047475No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAGAGACT330.15542577241899022No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTTAAAAGCAGAGACT330.15542577241899022No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC320.15071590052750566No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAGAAGCAGAGACT310.1460060286360211No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGGCTT300.14129615674453655No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTAAAAGCAGGGACTT300.14129615674453655No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTTAAAAGCAGAGACT290.136586284853052No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGTTAAAAGCAGAGACT290.136586284853052No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGCAGAGAC280.13187641296156744No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATCTAGTAAAAGCAGGGACTT270.1271665410700829No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC270.1271665410700829No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT270.1271665410700829No Hit
AGCCTAGACATCCACTGCTGGTGTCTCGTGGTAATGATCCTTCCGCTGTC270.1271665410700829No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGAATT250.1177467972871138No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC250.1177467972871138No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAGGCAGGGACTT240.11303692539562923No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTTAAAAGCAGAGACT240.11303692539562923No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGCAGAGAC240.11303692539562923No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC240.11303692539562923No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAATAAAAGCAGGGACTT230.10832705350414468No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAATTAAAAGCAGAGACT230.10832705350414468No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC230.10832705350414468No Hit
AGCCTAGACATCCACTGCTGATAGTTGCTATCTAGTAAAAGCAGGGACTT230.10832705350414468No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGCAGAGAC220.10361718161266015No Hit
AGCCTAGACATCCACTGCTGAAAGCTGCTATCTAGTAAAAGCAGGGACTT220.10361718161266015No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC220.10361718161266015No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGGCT220.10361718161266015No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGCAGAGAC220.10361718161266015No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC220.10361718161266015No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)