FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi681.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi681.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15423
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC505432.769240744342866No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT457029.63107047915451No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT179311.625494391493223No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT5503.5661025740776764No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC3422.2174674187901187No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTATCTAGAATAAAAGTAGAGACT1150.7456396291253323No Hit
AGCCTAGACATCCACTGCTGACGAGTGGTAATGATCCTTCCGCTGTCTCT610.39551319457952405No Hit
AGCCTAGACATCCACTGCTGTCTGTGGTAATGATCCTTCCGCTGTCTCTT550.35661025740776764No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGTAGAGAC370.23990144589249823No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGCGAACGCAAAAACTTT360.23341762303053878No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGAGAACGCAAAAACTTT360.23341762303053878No Hit
AGCCTAGACATCCACTGCTGCCGGTGGTAATGATCCTTCCGCTGTCTCTT350.2269338001685794No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT340.22044997730662No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC290.18803086299682292No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACACAAAAACTTT290.18803086299682292No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC290.18803086299682292No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC280.1815470401348635No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACTCAAAAACTTT280.1815470401348635No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC270.17506321727290408No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACCTT250.16209557154898527No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC240.15561174868702587No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC240.15561174868702587No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC230.14912792582506645No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCGAAAACTTT230.14912792582506645No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC230.14912792582506645No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAGACTTT220.14264410296310703No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT220.14264410296310703No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC220.14264410296310703No Hit
AGCCTAGACATCCACTGCTGGAAGTTTGTGGAAGTGAACGCAAAAACTTT210.13616028010114764No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT210.13616028010114764No Hit
AGCCTAGACATCCACTGCTAAAAGTTTGTGGAAGTGAACGCAAAAACTTT210.13616028010114764No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC200.12967645723918822No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGGAGTGAACGCAAAAACTTT200.12967645723918822No Hit
AGCCTAGACATCCACTGCTGATGTGGTAATGATCCTTCCGCTGTCTCTTA200.12967645723918822No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC200.12967645723918822No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC190.12319263437722881No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTC190.12319263437722881No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT180.11670881151526939No Hit
AGCCTAGACATCCACTGCTGAAAGTTCGTGGAAGTGAACGCAAAAACTTT180.11670881151526939No Hit
AGCCTAGACATCCACTGCTGAGAGTTTGTGGAAGTGAACGCAAAAACTTT170.11022498865331No Hit
AGCCTAGACATCCACTGCTGAAAGCTTGTGGAAGTGAACGCAAAAACTTT170.11022498865331No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC160.10374116579135058No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC160.10374116579135058No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGTAGAGAC160.10374116579135058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)