FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOChi677.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOChi677.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3933
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT77519.705059750826344No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT61915.73862191711162No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT43110.95855580981439No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT2556.483600305110603No Hit
AGCCTAGACATCCACTGCTGGAGATGTGGTAATGATCCTTCCGCTGTCTC1594.0427154843630815No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC1253.1782354436816678No Hit
AGCCTAGACATCCACTGCTGATGTGGTAATGATCCTTCCGCTGTCTCTTA1112.822273073989321No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT1092.7714213068904145No Hit
AGCCTAGACATCCACTGCTGGGTCTCGTGGTAATGATCCTTCCGCTGTCT1072.7205695397915077No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC711.8052377320111872No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT671.703534197813374No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT611.5509788965166538No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTTAGGATTAAAACCAAAGACTT561.4238494787693874No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTGTCTAGTTAAAAGCAGAGACT511.2967200610221206No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTGGATTAAAACCAAGACTTT421.0678871090770405No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT390.9916094584286803No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC360.9153318077803204No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAAGACT300.7627765064836003No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTGGATTAAAACTGTAGACTT280.7119247393846937No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTAGAAGTGAACGCAAAAACTTT270.6864988558352403No Hit
AGCCTAGACATCCACTGCTGTCGCGCACAGTGTGTATAGAGACAGTGGTA180.4576659038901602No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT130.33053648614289344No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTTAAAAGCAAGGACT120.30511060259344014No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT120.30511060259344014No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAGAGCAAGGACT70.17798118484617342No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGGACT70.17798118484617342No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAGAAGCAAGGACT60.15255530129672007No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTTAAAAGCAGAGACT50.12712941774726672No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATCTAGTTAAAAGCAGAGACT50.12712941774726672No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGTTAAAAGCAAGGACT50.12712941774726672No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAAGGACT50.12712941774726672No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAAACT40.10170353419781336No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTGAAAGCAGAGACT40.10170353419781336No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT40.10170353419781336No Hit
AGCCTAGACATCCACTGCTGAAAGTCGCTATCTAGTTAAAAGCAGAGACT40.10170353419781336No Hit
AGCCTAGACATCCGCTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC40.10170353419781336No Hit
ATCGGACACGTTCAACCATAGGCTCCATGATATCTGAAGAGCTAGTAAAC40.10170353419781336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)