FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH660.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH660.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12456
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT525642.19653179190752No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT379230.443159922928707No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT3312.657353885677585No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC2672.1435452793834298No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC2331.8705844572896597No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT830.6663455362877329No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT750.6021194605009634No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAATTAAAAGCAAGAATT480.3853564547206166No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATC330.26493256262042386No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTAAGAGCAAGGACTT320.2569043031470777No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATTTAATTAAAAGCAAGAATT310.2488760436737315No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGGGCAAGGACTT310.2488760436737315No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAGTTAAAAGCAAGAATT290.23281952472703915No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTAAGAGCAAGGACTT280.22479126525369297No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTAAGAGCAAGGACTT270.2167630057803468No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTAAGAGCAAGGACTT250.2007064868336545No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTC250.2007064868336545No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTAAGAGCAAGGACTT250.2007064868336545No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAGGGACTT250.2007064868336545No Hit
AGCCTAGACATCCACTGCTGCCGACGGTGGTAATGATCCTTCCGCTGTCT250.2007064868336545No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAATAAGAGCAAGGACTT240.1926782273603083No Hit
AGCCTAGACATCCACTGCTGAGAGTTGCTATTTAATTAAAAGCAAGAATT240.1926782273603083No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTAAGAGCAAGGACTT230.1846499678869621No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAGAGCAAGGACTT220.17662170841361594No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTAAGAGCAAGGACTT220.17662170841361594No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTACTTAAAAGCAAGAATT210.16859344894026976No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACCT210.16859344894026976No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT200.16056518946692355No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGTAAGAGCAAGGACTT200.16056518946692355No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAACTAAAAGCAAGAATT200.16056518946692355No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGGCTT190.15253692999357737No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAGGAGCAAGGACTT190.15253692999357737No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCGAGGACTT180.1445086705202312No Hit
CGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT180.1445086705202312No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT180.1445086705202312No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTATTTAAAAGCAAGAATT170.13648041104688502No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTGAGAGCAAGGACTT160.12845215157353884No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAGGCAAGAATT160.12845215157353884No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAGGAATT160.12845215157353884No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGGATT160.12845215157353884No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTACTTAATTAAAAGCAAGAATT160.12845215157353884No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGTAAGAGCAAGGACTT160.12845215157353884No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAGAGCAAGAATT150.12042389210019266No Hit
AGCCTATACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT150.12042389210019266No Hit
AGCCTAGACATCCACTGCTGACAGTTGTTATCTAGTAAGAGCAAGGACTT150.12042389210019266No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAGTT150.12042389210019266No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGTAAGAGCAAGGACTT140.11239563262684649No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATTTAATTAAAAGCAAGAATT140.11239563262684649No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCAAGAGCAAGGACTT130.10436737315350034No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAATAGCAAGAATT130.10436737315350034No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTGATTAAAAGCAAGAATT130.10436737315350034No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)