FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH657.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH657.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT87157.491749174917494No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC45930.297029702970296No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGAGAACGCAAAAACTTT120.7920792079207921No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT50.33003300330033003No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC40.264026402640264No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGGGAACGCAAAAACTTT40.264026402640264No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT40.264026402640264No Hit
AGCCTAGACATCCACTGCTGAAAGTTTTTGGAAGTGAACGCAAAAACTTT40.264026402640264No Hit
AGCCAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC30.19801980198019803No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAGAAACTTT30.19801980198019803No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT30.19801980198019803No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAATGAACGCAAAAACTTT30.19801980198019803No Hit
AGCCTAGACATCCACTGCTAAAAGTTTGTGGAAGTGAACGCAAAAACTTT30.19801980198019803No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC30.19801980198019803No Hit
AGCCTAGCATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC20.132013201320132No Hit
AGCCTAGATATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT20.132013201320132No Hit
AGCCTAGACATCCACTGCTGAAAGCTTGTGGAAGTGAACGCAAAAACTTT20.132013201320132No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGCGAACGCAAAAACTTT20.132013201320132No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACAATAAAAGCAGAGAC20.132013201320132No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAAAATAAAAGCAGAGAC20.132013201320132No Hit
AGCCTAGACATCCACTGCTGAAAGTTTATGGAAGTGAACGCAAAAACTTT20.132013201320132No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTAGAAGTGAACGCAAAAACTTT20.132013201320132No Hit
AGCCTAGACTCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC20.132013201320132No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC20.132013201320132No Hit
AGCCTAGACACCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC20.132013201320132No Hit
AGCCTAGACATTCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGA20.132013201320132No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAGACTTT20.132013201320132No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT20.132013201320132No Hit
AGCCTAGACATCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC20.132013201320132No Hit
AGCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC20.132013201320132No Hit
AGCCTAGACATCCACTGCTGAAAGTTCGTGGAAGTGAACGCAAAAACTTT20.132013201320132No Hit
AGCCTAGACATCTACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT20.132013201320132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)