FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH655.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH655.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3155
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT276687.6703645007924No Hit
AGCCTAGACATCCACTGCTGACGACGACGTGGTAATGATCCTTCCGCTGT240.7606973058637084No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGAGAACGCAAAAACTTT240.7606973058637084No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGCGAACGCAAAAACTTT150.4754358161648178No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC100.31695721077654515No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT100.31695721077654515No Hit
AGCCTAGACATCCACTGCTAAAAGTTTGTGGAAGTGAACGCAAAAACTTT90.28526148969889065No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT80.25356576862123614No Hit
AGCCTAGACATCTACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT80.25356576862123614No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAAATTT70.22187004754358164No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGGGAACGCAAAAACTTT70.22187004754358164No Hit
AGCCTAGACATCCACTGCTGACAGTTTGTGGAAGTGAACGCAAAAACTTT70.22187004754358164No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT70.22187004754358164No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAATGAACGCAAAAACTTT70.22187004754358164No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACTCAAAAACTTT60.1901743264659271No Hit
AGCCTAGACATCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC60.1901743264659271No Hit
AGCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC50.15847860538827258No Hit
AGCCTAGACATCCACTGCTGAAAGTTGGTGGAAGTGAACGCAAAAACTTT40.12678288431061807No Hit
AGCCTAGACATCCACTGCTGAAAGTTTTTGGAAGTGAACGCAAAAACTTT40.12678288431061807No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACACAAAAACTTT40.12678288431061807No Hit
AGCCTAAACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT40.12678288431061807No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT40.12678288431061807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)