FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH654.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH654.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5064
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT335566.25197472353871No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC99919.72748815165877No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGAGAACGCAAAAACTTT270.533175355450237No Hit
AGACTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT150.2962085308056872No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGCGAACGCAAAAACTTT140.2764612954186414No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGCGGAAGTGAACGCAAAAACTTT110.21721958925750395No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT110.21721958925750395No Hit
AGCCTAGACATCCACTGCTAAAAGTTTGTGGAAGTGAACGCAAAAACTTT110.21721958925750395No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT100.1974723538704581No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTCT90.17772511848341233No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT90.17772511848341233No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT90.17772511848341233No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC80.1579778830963665No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAGAACTTT80.1579778830963665No Hit
AGCCTAGACATCCACTGCTGACAGTTTGTGGAAGTGAACGCAAAAACTTT80.1579778830963665No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT80.1579778830963665No Hit
AGCCTAGACATCCACTGCTGAAAGTTCGTGGAAGTGAACGCAAAAACTTT80.1579778830963665No Hit
CGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT70.1382306477093207No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACACAAAAACTTT70.1382306477093207No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC70.1382306477093207No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTC70.1382306477093207No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACTCAAAAACTTT70.1382306477093207No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGGGAACGCAAAAACTTT70.1382306477093207No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC60.11848341232227488No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAGACTTT60.11848341232227488No Hit
AGCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC60.11848341232227488No Hit
AGCCTAGACATCCACTGCTGAAAGCTTGTGGAAGTGAACGCAAAAACTTT60.11848341232227488No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGGAGTGAACGCAAAAACTTT60.11848341232227488No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC60.11848341232227488No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)