FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH653.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH653.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2444
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC89736.702127659574465No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT35614.566284779050736No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT33613.747954173486088No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATGGATTCAAACCAAAGACTT2299.36988543371522No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT1104.500818330605565No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC873.5597381342062198No Hit
AGCCTAGACATCCACTGCTGTCGTGGCAGCGTCAGATGTGGTAATGATCC120.4909983633387889No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC90.36824877250409166No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGCAGAGAC50.20458265139116205No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC50.20458265139116205No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC50.20458265139116205No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC50.20458265139116205No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGCAGAGAC50.20458265139116205No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGCAGAGAC40.16366612111292964No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC40.16366612111292964No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC40.16366612111292964No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGTAAAAGCAAGGACTT40.16366612111292964No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC30.12274959083469722No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC30.12274959083469722No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTC30.12274959083469722No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTAAAAGCAAGGACTT30.12274959083469722No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAATAAGAGCAAGGACTT30.12274959083469722No Hit
AGCCTAGACATCCACTGCTGACAGTTGTTATCTAGAATAAAAGCAGAGAC30.12274959083469722No Hit
AGCCTAGACATCCACTGCTGGCCTCGTGGTAATGATCCTTCCGCTGTCTC30.12274959083469722No Hit
AGCCTATACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC30.12274959083469722No Hit
AGCCTAGACATCCACTGCTGAAAGATGTTATCTAGTAAAAGCAAGGACTT30.12274959083469722No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGAGACTT30.12274959083469722No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT30.12274959083469722No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGCAGAGAC30.12274959083469722No Hit
AGCCTAGACATCCACTGCTGTCGTGGCAGCGTCAGGTGTGGTAATGATCC30.12274959083469722No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTAAAAGCAAGGACTT30.12274959083469722No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)