FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH651.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH651.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13731
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT621245.24069623479717No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT401029.20399096933945No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT5113.7215060811302894No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC1370.9977423348627193No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC1140.8302381472580291No Hit
AGCCTAGACATCCACTGCTGGTGGTAATGATCCTTCCGCTGTCTCTTATA830.6044716335299687No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACCT470.3422911659748016No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTAAGAGCAAGGACTT450.32772558444395894No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTC410.29859442138227366No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGGGCAAGGACTT370.26946325832058843No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTAAGAGCAAGGACTT360.26218046755516716No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAATAAGAGCAAGGACTT350.25489767678974584No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAGAGCAAGGACTT340.24761488602432452No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGTAAGAGCAAGGACTT340.24761488602432452No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTAAGAGCAAGGACTT320.23304930449348193No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACC310.2257665137280606No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTTAAAAGCAAGGACT310.2257665137280606No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAGGGACTT290.21120093219721797No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTTAAAAGCAAGGACT280.20391814143179668No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGTAAGAGCAAGGACTT270.19663535066637536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAGGAGCAAGGACTT270.19663535066637536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTAAGAGCAAGGACTT260.18935255990095404No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTAAGAGCAAGGACTT250.18206976913553274No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTAAGAGCAAGGACTT250.18206976913553274No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAAGGACT240.17478697837011142No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT230.16750418760469013No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGGACT230.16750418760469013No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTAAGAGCAAGGACTT220.1602213968392688No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGGCT200.14565581530842617No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCGAGGACTT200.14565581530842617No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAGAGCAAGGACT200.14565581530842617No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT190.13837302454300487No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTTAAAAGCAAGGACT170.12380744301216226No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT170.12380744301216226No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT170.12380744301216226No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAACAAGGACTT160.11652465224674097No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTTAAAAGCAAGGACT160.11652465224674097No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTTAAAAGCAAGGACT160.11652465224674097No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT150.10924186148131965No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT150.10924186148131965No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGTAAGAGCAAGGACTT150.10924186148131965No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAATTAAAAGCAAGGACT140.10195907071589834No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGTTAAAAGCAAGGACT140.10195907071589834No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGGCTT140.10195907071589834No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)