FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH648.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH648.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15039
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT637142.36318904182459No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT388025.79958773854645No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT10947.274419841744797No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT10667.088237249817142No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT890.591794667198617No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT610.4056120752709622No Hit
AGCCTAGACATCCACTGCTGGTGTCTCGTGGTAATGATCCTTCCGCTGTC560.37236518385530953No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATCTAGTTAAAAGCAGAGACT360.23937761819269898No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT360.23937761819269898No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACC330.21942948334330742No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTACCTAGTTAAAAGCAGAGACT330.21942948334330742No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT290.1928319702107853No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTATTTAAAAGCAGAGACT290.1928319702107853No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGAGAACGCAAAAACTTT290.1928319702107853No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTTAAAAGCAGAGACT260.1728838353613937No Hit
AGCCTAGACATCCACTGCTAAAAGTTTGTGGAAGTGAACGCAAAAACTTT240.15958507879513265No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT230.15293570051200212No Hit
AGCCTAGACATCCACTGCTGAAAGTCGCTATCTAGTTAAAAGCAGAGACT230.15293570051200212No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGCGAACGCAAAAACTTT220.1462863222288716No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAGGCAGAGACT220.1462863222288716No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAAACT220.1462863222288716No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAATTAAAAGCAGAGACT220.1462863222288716No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGGCT210.13963694394574108No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC210.13963694394574108No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAGAAGCAGAGACT200.13298756566261055No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCCATCTAGTTAAAAGCAGAGACT200.13298756566261055No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGCGGAAGTGAACGCAAAAACTTT200.13298756566261055No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCGGAGACT190.12633818737948002No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAGAAACTTT190.12633818737948002No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCCAGTTAAAAGCAGAGACT180.11968880909634949No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT170.11303943081321897No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAGACTTT170.11303943081321897No Hit
AGCCTAGACATCCACTGCTGAGAGTTTGTGGAAGTGAACGCAAAAACTTT170.11303943081321897No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT170.11303943081321897No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGCTAAAAGCAGAGACT160.10639005253008844No Hit
CGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT160.10639005253008844No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTACTTAAAAGCAGAGACT160.10639005253008844No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGGGACT160.10639005253008844No Hit
AGCCTAGACATCCACTGCTGAAAGCTGCTATCTAGTTAAAAGCAGAGACT160.10639005253008844No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)