FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH647.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH647.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17362
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT1426282.14491418039397No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC4662.6840225780440043No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGAGAACGCAAAAACTTT1150.6623660868563529No Hit
AGCCTAGACATCCACTGCTAAAAGTTTGTGGAAGTGAACGCAAAAACTTT830.47805552355719383No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGCGAACGCAAAAACTTT790.455016703144799No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT530.3052643704642322No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT520.2995046653611335No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT520.2995046653611335No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT490.28222555005183736No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT470.2707061398456399No Hit
AGCCTAGACATCCACTGCTGAAAGTTTTTGGAAGTGAACGCAAAAACTTT460.26494643474254115No Hit
CGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT430.24766731943324502No Hit
AGCCTAGACATCCACTGCTGAAAGTTCGTGGAAGTGAACGCAAAAACTTT410.23614790922704756No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGCGGAAGTGAACGCAAAAACTTT390.22462849902085014No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGGGAACGCAAAAACTTT390.22462849902085014No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAGACTTT360.20734938371155398No Hit
AGCCTAGACATCCACTGCTGAAAGTTGGTGGAAGTGAACGCAAAAACTTT330.1900702684022578No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGCAGTGAACGCAAAAACTTT330.1900702684022578No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTC300.17279115309296164No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACACAAAAACTTT290.1670314479898629No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACCTT290.1670314479898629No Hit
AGCCTAGACATCCACTGCTGACAGTTTGTGGAAGTGAACGCAAAAACTTT290.1670314479898629No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAGAACTTT280.16127174288676419No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAGAAACTTT280.16127174288676419No Hit
AGCCTAGACATCCACTGCTGAAAGTCTGTGGAAGTGAACGCAAAAACTTT280.16127174288676419No Hit
AGCCTAGACATCCACTGCTGAAAGCTTGTGGAAGTGAACGCAAAAACTTT280.16127174288676419No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTCT270.15551203778366546No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACTCAAAAACTTT270.15551203778366546No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAACAACTTT260.14975233268056676No Hit
AGCCTAGACATCCACTGCTGGAAGTTTGTGGAAGTGAACGCAAAAACTTT250.14399262757746803No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAATGCAAAAACTTT240.1382329224743693No Hit
AGCCTAAACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT220.12671351226817187No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCGAAAACTTT210.12095380716507313No Hit
AGTCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT200.11519410206197443No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGGAGTGAACGCAAAAACTTT200.11519410206197443No Hit
AGACTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT180.10367469185577699No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAAATTT180.10367469185577699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)