FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH643.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH643.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18991
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT1630985.8775209309673No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGAGAACGCAAAAACTTT1460.7687852140487599No Hit
AGCCTAGACATCCACTGCTAAAAGTTTGTGGAAGTGAACGCAAAAACTTT730.38439260702437994No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT700.36859565057132326No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGCGAACGCAAAAACTTT490.2580169553999263No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAGCGCAAAAACTTT460.24221999894686957No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT440.23168869464483174No Hit
AGCCTAGACATCCACTGCTGAAAGTTGGTGGAAGTGAACGCAAAAACTTT420.22115739034279397No Hit
AGCCTAGACATCCACTGCTGAAAGTTTTTGGAAGTGAACGCAAAAACTTT410.21589173819177507No Hit
CGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT360.18956347743668053No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACCTT330.17376652098362383No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGCGGAAGTGAACGCAAAAACTTT330.17376652098362383No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACTCAAAAACTTT330.17376652098362383No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGGGAACGCAAAAACTTT330.17376652098362383No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGCAGTGAACGCAAAAACTTT290.15270391237954822No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTC280.1474382602285293No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAGGTGAACGCAAAAACTTT270.1421726080775104No Hit
AGCCTAGACATCCACTGCTGAAGGTTTGTGGAAGTGAACGCAAAAACTTT270.1421726080775104No Hit
AGCCTAGACATCCACTGCTGACAGTTTGTGGAAGTGAACGCAAAAACTTT270.1421726080775104No Hit
AGCCTATACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT250.1316413037754726No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAAATTT240.1263756516244537No Hit
AGACTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT230.12110999947343479No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAATGAACGCAAAAACTTT230.12110999947343479No Hit
AGCCTAGACATCCACTGCTGAAAGTCTGTGGAAGTGAACGCAAAAACTTT220.11584434732241587No Hit
AGCCTAGACATCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC220.11584434732241587No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTAGAAGTGAACGCAAAAACTTT210.11057869517139698No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAGAAACTTT210.11057869517139698No Hit
AGCCTAGACATCCACTGCTGAAAGTTCGTGGAAGTGAACGCAAAAACTTT210.11057869517139698No Hit
AGTCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT200.10531304302037808No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAGACTTT200.10531304302037808No Hit
AGCCTAGACATCCACTGCTGAACGTTTGTGGAAGTGAACGCAAAAACTTT200.10531304302037808No Hit
AGCCTAGATATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT190.10004739086935917No Hit
AGCCTAGACATTCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT190.10004739086935917No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)