FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH639.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH639.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17925
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT870948.58577405857741No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT232912.993026499302651No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT15528.658298465829846No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT14588.133891213389122No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC4402.4546722454672243No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT1570.8758716875871687No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT960.5355648535564854No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATTGATTAAAACCAAAGACTT730.40725244072524414No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGAAAGGACTT700.3905160390516039No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGAGTAAAACCAAAGACTT690.38493723849372385No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACCT560.3124128312412831No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAGCCAAAGACTT530.29567642956764295No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATTGATTAAAACCAAAGACTT490.2733612273361227No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT450.2510460251046025No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAGGACTT440.24546722454672243No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGAATAAAACCAAAGACTT410.2287308228730823No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATTGATTAAAACCAAAGACTT380.21199442119944212No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGACTAAAACCAAAGACTT370.20641562064156208No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAGAACCAAAGACTT350.19525801952580196No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGGCTT340.1896792189679219No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCGATTAAAACCAAAGACTT340.1896792189679219No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTAATTAAAACCAAAGACTT340.1896792189679219No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATTGATTAAAACCAAAGACTT310.17294281729428174No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATTGATTAAAACCAAAGACTT300.16736401673640167No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTGATTAAAACCAAAGACTT290.16178521617852162No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTC290.16178521617852162No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGGTTAAAACCAAAGACTT270.1506276150627615No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATTGATTAAAACCAAAGACTT250.1394700139470014No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAGACCAAAGACTT230.12831241283124128No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT210.11715481171548119No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTGAAACCAAAGACTT210.11715481171548119No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCGAAGACTT210.11715481171548119No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAGAGACTT210.11715481171548119No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTTATTAAAACCAAAGACTT200.11157601115760112No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTTGATTAAAACCAAAGACTT190.10599721059972106No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACTGATTAAAACCAAAGACTT190.10599721059972106No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)