FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH628.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH628.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10695
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT536550.16362786348761No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT136912.800374006545114No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT135712.688172043010754No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC2492.3281907433380082No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC2011.879382889200561No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTCAAAAGCAGAGACT1651.5427769985974753No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT1071.0004675081813932No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT670.6264609630668536No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTATTTAAAAGCAGAGACT310.2898550724637681No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTTAAAAGCAGAGACT290.2711547452080411No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTACCTAGTTAAAAGCAGAGACT290.2711547452080411No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTTAAAAGCAGAGACT280.26180458158017766No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACC270.25245441795231416No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGGCT240.22440392706872372No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCGGAGACT230.21505376344086022No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTGTCTAGTTAAAAGCAGAGACT230.21505376344086022No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATCTAGTTAAAAGCAGAGACT220.2057035998129967No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAGGCAGAGACT220.2057035998129967No Hit
AGCCTAGACATCCACTGCTGAGAGTTGCTATCTAGTTAAAAGCAGAGACT220.2057035998129967No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT210.19635343618513326No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAATTAAAAGCAGAGACT210.19635343618513326No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAGAGCAAGGACTT180.16830294530154277No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTGGTTAAAAGCAGAGACT180.16830294530154277No Hit
AGCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACTT170.1589527816736793No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGAGAACGCAAAAACTTT160.14960261804581582No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAGAAGCAGAGACT150.1402524544179523No Hit
CGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT150.1402524544179523No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAAACT150.1402524544179523No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGGGACT150.1402524544179523No Hit
AGCCTATACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT140.13090229079008883No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCCAGTTAAAAGCAGAGACT130.12155212716222534No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT130.12155212716222534No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCCATCTAGTTAAAAGCAGAGACT120.11220196353436186No Hit
AGCCTAGACATCCACTGCTGAGAGTTTGTGGAAGTGAACGCAAAAACTTT120.11220196353436186No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCAAGTTAAAAGCAGAGACT120.11220196353436186No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTACTTAAAAGCAGAGACT110.10285179990649836No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGAAGAGACT110.10285179990649836No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGGACGCAAAAACTTT110.10285179990649836No Hit
AGCCTAGACATCCACTGCTTAAAGTTGCTATCTAGTTAAAAGCAGAGACT110.10285179990649836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)