FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH625.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH625.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12981
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT703654.20229566289192No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAAGACTT303423.372621523765503No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT7325.639010862029119No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTGGATTAAAACCAAGACTTT950.7318388413835606No Hit
AGCCTAGACATCCACTGCTGGATGTGGTAATGATCCTTCCGCTGTCTCTT610.46991757183576No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT610.46991757183576No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC610.46991757183576No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT490.37747477081888914No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT490.37747477081888914No Hit
AGCCTAGACATCCACTGCTGTAAGAGACAGTGGTAATGATCCTTCCGCTG460.35436407056467145No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT320.24651413604498884No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTT320.24651413604498884No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACCT310.2388105692935829No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTAAAAGCAAGGACTT290.22340343579077115No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAATTAAAACCAAGAACT270.20799630228795934No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAAGACTC260.2002927355365534No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTAAAAGCAAGGACTT240.18488560203374163No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGGCTT240.18488560203374163No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTAAAAGCAAGGACTT240.18488560203374163No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTAAAAGCAAGGACTT240.18488560203374163No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAATAAAAGCAAGGACTT230.17718203528233573No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAACCAAGAGAC220.16947846853092982No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAGGCAAGGACTT220.16947846853092982No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTAAAAGCAAAGACTT210.16177490177952392No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTAAAAGCAAGGACTT200.154071335028118No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTAAAAGCAAGGACTT170.13096063477390033No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTC170.13096063477390033No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGTAAAAGCAAGGACTT170.13096063477390033No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAGAAGCAAGGACTT160.12325706802249442No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAACAAGGACTT160.12325706802249442No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGTAAAAGCAAGGACTT150.11555350127108852No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGTAAAAGCAAGGACTT150.11555350127108852No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTCATCTAGTAAAAGCAAGGACTT150.11555350127108852No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTAAAAGCAAGGACTT140.10784993451968261No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACTAAAAGCAAGGACTT130.1001463677682767No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGTAAAAGCAAAGACTT130.1001463677682767No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGAACTT130.1001463677682767No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGTAAAAGCAAGGACTT130.1001463677682767No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT130.1001463677682767No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)