FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH622.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH622.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC11947.410358565737056No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT7429.482071713147413No Hit
AGCCTAGACATCCACTGCTGAGTGTGGTAATGATCCTTCCGCTGTCTCTT114.382470119521913No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT103.9840637450199203No Hit
AGCCTAGACATCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT31.1952191235059761No Hit
AGCCTAGACATACACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC20.796812749003984No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC20.796812749003984No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC20.796812749003984No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT10.398406374501992No Hit
AGCCTAGACATCCAATGCAGAAAGTTGTTATCTAGAATAAAAGCAGAGGC10.398406374501992No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACAATAAAAGCAGAGAC10.398406374501992No Hit
CGGCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGT10.398406374501992No Hit
AGCCTAGAAATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC10.398406374501992No Hit
AGCCTAGACATCCACTGCTGAAAGTTATTATCTAGAATAAAAGCAGAGAC10.398406374501992No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAAACCGAGACC10.398406374501992No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC10.398406374501992No Hit
GTACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTTAAAG10.398406374501992No Hit
CGGCTGCGTTCTTCATCGATGCGCAAGCCAAGAGATCCGTTGTTGAAAGT10.398406374501992No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT10.398406374501992No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGCAGTAAACGCAAAAACTTT10.398406374501992No Hit
AGCCTAGACATCCACTGCTAAAAGTTTGAGGAAGAGAACGCAAAAACTTT10.398406374501992No Hit
AGCCTAGACATCTACTGCTGAGTGTGGTAATGATCCTTCCGCTGTCTCTT10.398406374501992No Hit
TGCATACTGACCAAGTACGTGATTACTTCATGAAGCGTTACAATGATGTT10.398406374501992No Hit
AGCCTAGACATCCACTGCTGTCCCGTGGTAATGATCCTTCCGCTGTCTCT10.398406374501992No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGCAGAGAC10.398406374501992No Hit
AGCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTTC10.398406374501992No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATAATCCTTCCGCTGTCTCT10.398406374501992No Hit
AGCCTAGAAAATCACTGCTGAAAGTTTGTAGAAGATACCGCAAAAACTTT10.398406374501992No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC10.398406374501992No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC10.398406374501992No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTTAAAAGCAAGGACT10.398406374501992No Hit
CGGCTGCGTTCTTCATCGATGCGAGAGCCAAGAGTTCCGTTGTTGAAACT10.398406374501992No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGACCGCAAAAACTTT10.398406374501992No Hit
AACCTAGACCTCCACTGCTGAAAGTTTGTGGAATTGACCGCACAACCTAT10.398406374501992No Hit
AGCCTAGACATCCACTGCTGAGTGTGGTAATGATGCTTCCGCTGTCTCTT10.398406374501992No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT10.398406374501992No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)