FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH620.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH620.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19223
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT604831.462310773552517No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT549428.58034645996983No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC231812.058471622535505No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT10055.228112157311554No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT6143.194090412526661No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTCAAAAGCAGAGACT1730.8999635852884565No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAGAGCAAGGACTT560.2913176923477085No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT460.23929667585704623No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAGGCAGAGACT400.20808406596264895No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTC360.18727565936638402No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC350.18207355771731779No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGGCT350.18207355771731779No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT330.17166935441918535No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTTAAAAGCAGAGACT310.1612651511210529No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACC310.1612651511210529No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGGGACT310.1612651511210529No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTATTTAAAAGCAGAGACT290.15086094782292048No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAGGGACTT280.14565884617385425No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTAAGAGCAAGGACTT270.140456744524788No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCCATCTAGTTAAAAGCAGAGACT270.140456744524788No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATCTAGTTAAAAGCAGAGACT270.140456744524788No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTAAGAGCAAGGACTT250.13005254122665555No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTAAGAGCAAGGACTT240.12485043957758934No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAATAAGAGCAAGGACTT240.12485043957758934No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGGCTT240.12485043957758934No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTTAAAAGCAGAGACT240.12485043957758934No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTAAGAGCAAGGACTT230.11964833792852311No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC230.11964833792852311No Hit
AGCCTAGACATCCACTGCTGAAAGTCGCTATCTAGTTAAAAGCAGAGACT230.11964833792852311No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAGGAGCAAGGACTT230.11964833792852311No Hit
AGCCTAGACATCCACTGCTGAAAGCTGCTATCTAGTTAAAAGCAGAGACT230.11964833792852311No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT220.11444623627945691No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGGGCAAGGACTT220.11444623627945691No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAATTAAAAGCAGAGACT220.11444623627945691No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGCTAAAAGCAGAGACT210.10924413463039068No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTAAGAGCAAGGACTT200.10404203298132447No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCGGAGACT200.10404203298132447No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTACCTAGTTAAAAGCAGAGACT200.10404203298132447No Hit
AGCCCAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT200.10404203298132447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)