FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH616.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH616.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10012
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC732573.1622053535757No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT6506.492209348781462No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT750.7491010787055533No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGTAGAGAC670.6691969636436277No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATGGATTAAAACCAAAAACTT450.449460647223332No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGTAGAGAC430.4294846184578506No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGTAGAGAC420.4194966040751098No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGGGAC410.40950858969236914No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGTAGAGAC410.40950858969236914No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGAATAAAAGTAGAGAC390.38953256092688776No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGTAGAGAC370.3695565321614063No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGGC360.3595685177786656No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGTAGAGAC320.31961646024770274No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAAAATAAAAGTAGAGAC310.309628445864962No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGTAGAGAC310.309628445864962No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGTAGAGAC300.2996404314822213No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGTAGAGAC300.2996404314822213No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAGAGTAGAGAC300.2996404314822213No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC290.28965241709948064No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC290.28965241709948064No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGTAGAGAC280.2796644027167399No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAATT270.2696763883339992No Hit
AGCCTAGACATCCACTGCTGTGGTAATGATCCTTCCGCTGTCTCTTATAC270.2696763883339992No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAAAGAC260.2596883739512585No Hit
AGCCTAGACATCCACTGCTGAAAGTTATTATTGATTAAACAGACGACTTT210.2097483020375549No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGAATAAAAGTAGAGAC200.1997602876548142No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGAATAAAAGTAGAGAC200.1997602876548142No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGTAGAGAC200.1997602876548142No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT200.1997602876548142No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT170.1697962445065921No Hit
AGCCTAGACATCCACTGCTGACAGTTGTTATCTAGAATAAAAGTAGAGAC160.15980823012385137No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTGGAGAC160.15980823012385137No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGTAGAGAC160.15980823012385137No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATGAAAGTAGAGAC150.14982021574111065No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACAATAAAAGTAGAGAC150.14982021574111065No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCGAGAATAAAAGTAGAGAC150.14982021574111065No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAT150.14982021574111065No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC130.12984418697562924No Hit
AGCCTATACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC130.12984418697562924No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGTAGAGAC120.11985617259288853No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC110.10986815821014781No Hit
AGCCTAGACATCCACCGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC110.10986815821014781No Hit
AGCCTAGACATCCACTGCTGAACGTTGTTATCTAGAATAAAAGTAGAGAC110.10986815821014781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)